Widefield

The Wolfson Bioimaging Facility has 6 widefield microscopes offering routine fluorescence and transmitted light imaging alongside advanced applications including ratiometric analysis (e.g. FRET, calcium imaging), multi-well imaging and deconvolution

In widefield microscopy cameras are used to capture images equivalent to the view down the microscope eyepieces. When the additional features of more advanced imaging techniques such as CLSM (e.g. improved axial resolution and region of interest scanning) are not essential, use of widefield microscopes should be considered as they are easier, quicker and cheaper.

Features

All our widefields (except widefield 4) have:

  • inverted microscopes with some DIC and phase contrast lenses
  • sensitive monochrome cameras
  • incubators for temperature control
  • CO2 enrichment if required for live cell imaging.

Widefield 1 has a DV2 Multichannel imaging system which allows simultaneous capture of CFP and YFP or GPF and mCherry.

Widefield systems 2, 5, 6, and 7 all have motorised stages for multi-site imaging.

Widefield systems 4 and 5 both have colour CCD cameras (Widefield 5 also has a monochrome CCD camera on a separate port).

Widefield systems 5, 6 and 7 have adaptive focus control to correct for focus drift during time-lapse imaging.

Widefield 6 has a Photometrics Prime 95B sCMOS camera for sensitive high speed live cell imaging as well as improved resolution techniques such as super resolution radial fluctuation microscopy (SRRF) 

Full list of equipment
SystemRoomFurther information
Zeiss Z.1 lightsheet system B10C

Lightsheet technical specifications (PDF, 94kB) 

Further details

Deltavision
WFM with motorised stage, deconvolution and cell tracking. 

B10a Deltavision technical specifications (PDF, 90kB)

Widefield 1
Dual camera system with fast excitation and emission wavelength and focus control

Now has DV2 Multichannel imaging system which allows simultaneous capture of CFP and YFP or GPF and mCherry.

C27A

Widefield 1 technical specifications (PDF, 279kB)

User guide (PDF, 1,079kB)

Widefield 2
WFM with Leica DFC365FX camera

(restricted availability)

 B10A Widefield 2 technical specifications (PDF, 176kB)
Widefield 4
Inverted microscope with colour CCD camera and no incubator
C35 Widefield 4 technical specifications (PDF, 173kB)

Widefield 5
WFM with motorised stage, adaptive focus control, Leica DFC365FX and  DFC420C colour camera
B10A Widefield 5 technical specifications (PDF, 202kB)
Widefield 6
WFM with motorised stage, adaptive focus control, Photometrics Prime 95B sCMOS camera
B10A Widefield 6 technical specifications (PDF, 152kB)
Widefield 7
WFM with motorised stage, adaptive focus control, Andor Ixon EMCCD and Neo sCMOS cameras
C40A  Widefield 7 technical specifications (PDF, 214kB)

IncuCyte Zoom

Automated multi-site live cell imaging in phase contrast and fluorescence modes, multiple vessels, long-term imaging and high content analysis.

 C40 Incucyte Zoom technical specifications (PDF, 193kB)

More information and access

For further information or to arrange access to this equipment, contact one of the team.

We welcome comments or suggestions. Please contact one of the team or one of the steering group.

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