Correlative light electron microscopy (CLEM)
The Wolfson Bioimaging Facility is specifically designed to be able to perform Correlative Light Electron Microscopy (CLEM) experiments. CLEM combines the advantages of light microscopy (LM) and electron microscopy (EM) in one experiment. In LM one can for instance do live cell imaging and get a good overview of many cells, EM on the other hand provides much higher resolution and in addition will provide information about the surrounding space as an added bonus. By performing a CLEM experiment one should get more information out of the data then from either modality alone.
Although a lot of the workflows for CLEM have been simplified and streamlined it is still a technique that cannot easily be provided as a “service”. In most cases performing a CLEM project will be done in collaboration with the group of Dr. Paul Verkade. His group has a vast experience in a wide variety of CLEM technologies and is constantly improving and developing new approaches.
If you would like to discuss a CLEM approach for your biological question please contact Paul Verkade firstname.lastname@example.org.
Possibilities with CLEM
If you are interested in finding out more about CLEM, or to see if you can apply CLEM to your biological question, browse through some of our recent publications and books which highlight many of the possibilities of these exciting experiments.
- Methods in Cell Biology, Volume 124: Correlative Light and Electron Microscopy II. Shared with Thomas Müller-Reichert.
- Methods in Cell Biology, Volume 111: Correlative Light and Electron Microscopy. Shared with Thomas Müller-Reichert.
- Olmos, Y. L. Hodgson, J. Mantell, P. Verkade, and J.G. Carlton (2015). ESCRT-III controls nuclear envelope reformation. Nature, 522: 236–239
- Hodgson L, D. Nam, J. Mantell, A. Achim, and P. Verkade. (2014). Retracing in Correlative Light Electron Microscopy: Where is My Object of Interest? Methods in Cell Biology, Volume 124: Correlative Light and Electron Microscopy, 1-21.
- Hodgson L, J. Tavaré, and P. Verkade. (2014). Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking. Protoplasma. 251:403-416.
- Benito-Alifonso D, S. Tremel, B. Hou, H. Lockyear, J. Mantell, D.J. Fermin, P. Verkade, M. Berry, and M.C. Galan. (2014). Lactose as a "trojan horse" for quantum dot cell transport. Angew Chem Int Ed Engl. 53:810-814.
- Brown, E., J., Van Weering, T. Sharp, J. Mantell, and P. Verkade (2012). Capturing endocytic segregation events with HPF-CLEM. Methods in Cell Biology, Volume 111: Correlative Light and Electron Microscopy, 175-201.
- Brown, E. and P. Verkade (2010). The use of markers for Correlative Light Electron Microscopy. Protoplasma, 244: 91-97.
- Brown, E., J. Mantell, D.A. Carter, G. Tilly, and P. Verkade (2009). Studying intracellular transport using High-Pressure Freezing and Correlative Light Electron Microscopy. Seminars in Cell and Developmental Biology, 20: 910-919.
- P. Verkade. (2008). Moving EM: The Rapid Transfer System as a New Tool for Correlative Light and Electron Microscopy and High Throughput for High-Pressure Freezing. Journal of Microscopy. 230: 317-328.