Correlative light electron microscopy (CLEM)

The Wolfson Bioimaging Facility is specifically designed to be able to perform Correlative Light Electron Microscopy (CLEM) experiments. CLEM combines the advantages of light microscopy (LM) and electron microscopy (EM) in one experiment. In LM one can for instance do live cell imaging and get a good overview of many cells, EM on the other hand provides much higher resolution and in addition will provide information about the surrounding space as an added bonus. By performing a CLEM experiment one should get more information out of the data then from either modality alone.

Although a lot of the workflows for CLEM have been simplified and streamlined it is still a technique that cannot easily be provided as a “service”. In most cases performing a CLEM project will be done in collaboration with the group of Dr. Paul Verkade. His group has a vast experience in a wide variety of CLEM technologies and is constantly improving and developing new approaches.

If you would like to discuss a CLEM approach for your biological question please contact Paul Verkade p.verkade@bristol.ac.uk.

Possibilities with CLEM

If you are interested in finding out more about CLEM, or to see if you can apply CLEM to your biological question, browse through some of our recent publications and books which highlight many of the possibilities of these exciting experiments. 

Books

  • Methods in Cell Biology, Volume 124: Correlative Light and Electron Microscopy II. Shared with Thomas Müller-Reichert.
  • Methods in Cell Biology, Volume 111: Correlative Light and Electron Microscopy. Shared with Thomas Müller-Reichert.

Papers

More information

To discuss how a CLEM approach would work help with your biological question, contact Paul Verkade p.verkade@bristol.ac.uk.

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