The Facility houses a suite of electron microscopes covering a range of capabilities, including delivering high contrast images from biological material, cyro and electron tomography experiments, and a scanning electron microscope offering high and low vacuum, and environmental modes.
Tecnai 12 - FEI 120kV BioTwin SpiritTransmission Electron Microscope (TEM)
Delivering high contrast images from biological material.
Tecnai 20 - FEI 200kV Twin Lens Transmission Electron Microscope (TEM/STEM)
Offering cryo, tomography and STEM capability.
Quanta 200 - FEI Field Emission Scanning Electron Microscope
SEM with cyroSEM capability.
The facility houses both a FEI Vitrobot™ Mark IV and a Leica GP. Both are fully automated vitrification devices for plunge-freezing of aqueous (colloidal) suspensions.
The frozen specimens can then be analysed in the microscope using a Gatan CryoTransfer Specimen holder.
|High Pressure Freezer||Leica EMPACT2 + RTS.|
|Freeze Substitution||Leica AFS2 + FSP Leica AFS|
|Cryoultramicrotome||Leica EM UC7 with FC7 Cryo attachment|
|Ultramicrotomes||Leica EM UC6, Leica EM UC7 with IC80HD camera, Reichert Ultracut S.|
Most sample preparation can be done in the Unit including critical point drying and coating.