Cryo EM

In cryo-electron microscopy, specimens are rapidly frozen and maintained at liquid nitrogen temperatures. By avoiding the chemical fixation, dehydration and staining required for standard EM protocols, cryo-EM aims to preserve structures close to their native, hydrated state.

The Wolfson Bioimaging Facility provides cryo-SEM and cryo-TEM techniques that have been successfully applied to image chemical and biological material such as liposomes, viruses, and larger proteins.

In recent years, cryo-TEM image acquisition and single particle analysis have advanced rapidly to reveal protein structures at a resolution that was previously only achievable by X-ray crystallography. The GW4 Cryo-EM facility enables such applications with its FEI Talos Artica TEM and also supports advanced electron tomography of cell ultrastructure. The cryo-TEM (Tecnai 20) within the Wolfson Bioimaging Facility enables users to screen samples and obtain preliminary data prior to more detailed analysis on the higher specification Talos Artica cryo-TEM.    

 

For GW4 Cryo-EM information visit the webpage or contact the support team.

More information and access

For further information or to arrange access to this equipment, contact one of the team.

Please fill in this form to register sample processing requests.

We welcome comments or suggestions. Please contact one of the team or one of the steering group.

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