The Wolfson Bioimaging Facility is well equipped for a range of SEM experiments. Our user-friendly SEMs (FEI Quanta 200 FEG-SEM and Zeiss Evo 15 ESEM) ) are suitable for imaging biological samples and materials in a variety of modes.
- High vacuum SEM – typically used for biological materials following fixation, dehydration and coating with a thin layer of conductive metal (e.g. gold). This is also suitable for stable materials.
- Low vacuum / environmental SEM – to image materials in at least partially hydrated (and potentially living) states. This is also suitable for some non-conductive and low melting point materials
- Cryo-SEM (Quanta 200 only) – for cryo-fixed materials in fully hydrated state. Surfaces and fractured surfaces can be imaged.
The Zeiss Gemini 450 SBF-SEM is also available on request for high resolution high vacuum imaging.