Spinning disk

The Wolfson Bioimaging Facility offers spinning disk microscopy as an alternative form of confocal imaging with its Perkin Elmer UltraView and Opera systems.

Spinning disk microscopes offer an approach to confocal imaging which differs from more commonly used CLSM systems. In spinning disk systems, image detection by a camera is restricted to a single focal plane by an array of pinholes in a rapidly spinning disk which also distributes laser excitation across a field of view.

Improved optical efficiency and acquisition speed can offer advantages over CLSM systems for live imaging applications. Our  UltraView ERS 6FE spinning disk system (confocal 6) is equipped with 405, 440, 488, 514, 568 and 640nm laser lines, DIC optics, incubator and potential for CO2 enrichment.

Its functionality largely overlaps with the newer CLSM systems equipped for live imaging and is rarely used for imaging fixed samples, for which CLSM systems offer advantages in z-resolution, magnification range and sample dimensions.

Rapid focusing with a piezo device enables faster image stack acquisition than our CLSM systems and inclusion of a Photokinesis module facilitates spot bleaching during time-course imaging for interactive FRAP experiments.

The Opera LX system is a high content screening system built around a three laser spinning disk confocal microscope.

More information and access

For further information or to arrange access to this equipment, contact one of the team.

We welcome comments or suggestions. Please contact one of the team or one of the steering group

Edit this page