The Wolfson Bioimaging Facility offers spinning disk microscopy as an alternative form of confocal imaging with its high specification Perkin Elmer Ultraview system.
Spinning disk microscopes offer an approach to confocal imaging which differs from more commonly used CLSM systems. In spinning disk systems, image detection by a camera is restricted to a single focal plane by an array of pinholes in a rapidly spinning disk which also distributes laser excitation across a field of view.
Improved optical efficiency and acquisition speed can offer advantages over CLSM systems for live imaging applications. Our spinning disk microscope is a Perkin Elmer UltraView system equipped with 405, 440, 488, 514, 568 and 640nm laser lines, DIC optics, incubator and potential for CO2 enrichment.
Its functionality largely overlaps with the newer CLSM systems equipped for live imaging and is rarely used for imaging fixed samples, for which CLSM systems offer advantages in z-resolution, magnification range and sample dimensions.
Rapid focusing with a piezo device enables faster image stack acquisition than our CLSM systems and inclusion of a Photokinesis module facilitates spot bleaching during time-course imaging for interactive FRAP experiments.