Long term imaging

Live cell imaging is a well-established speciality of the Wolfson Bioimaging Facility.

Each light microscopy mode offered by the Wolfson Biomaging Facility can be applied to live cells and almost all of our microscopes are equipped with incubators and CO2-enrichment facilities to enable environmental control.

Imaging techniques that require intense illumination inevitably affect some cellular functions and at worst can be highly phototoxic. For this reason widefield microscopy has advantages for many live cell experiments and especially for long-term imaging.

In addition to traditional widefield microscopes we have a specialised automated live cell imaging system that has advantages for long-term and high content live cell imaging.

The IncuCyte ZOOM from Essen Biosciences is housed inside a CO2 incubator for optimised environmental stability. The system offers the following key features:

  • multi-sample imaging over hours and days
  • range of magnifications - 4x, 10x, 20x lenses
  • phase contrast imaging
  • fluorescence imaging - red and green
  • reproducible and repeated imaging of multiple fields - including whole well imaging
  • imaging of multi-well plates -up to 384-well
  • imaging of multiple vessels - up to 6 plates or combination of plates, flasks and dishes
  • software tools for batch analysis (‘high throughput’ imaging) of cell number, confluence, cell fluorescence and morphology
  • neuronal cell and dendrite analysis module
  • hardware and software tools for scratch wound migration assays in 96-well format.

Incucyte Zoom technical specifications (PDF, 193kB)

Recent papers

Hampton-O’Neil, Severn, Cross, Gurung, Nobes, Toye (2019) Ephrin/Eph receptor interaction facilitates macrophage recognition of differentiating human erythroblasts. Haematologica [Epub ahead of print]

Heideveld, Hampton-O'Neil, Cross, van Alphen, van den Biggelaar, Toye & van den Akker (2018). Glucocorticoids induce differentiation of monocytes towards macrophages that share functional and phenotypical aspects with erythroblastic island macrophages. Haematologica 103:395-405 

Liu, Mosienko, Vaccari Cardosa, Prokudina. Huentelman, Teschemacher, Kasparov (2018) Glio- and neuro-protection by prosaposin is mediated by orphan G-protein coupled receptors GPR37L1 and GPR37. Glia 66:2414-26

More information and access

For further information or to arrange access to this equipment, contact one of the team.

We welcome comments or suggestions. Please contact one of the team or one of the steering group.

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