Total internal reflection fluorescence (TIRF)
The Wolfson Bioimaging Facility provides total internal reflection fluorescence microscopy (TIRF) with its multi-laser, dual-camera Olympus/Abbelight SAFe360 system which also offers single molecule super-resolution imaging.
TIRF microscopy provides extremely high axial resolution by exciting the fluorophores with an evanescent field (caused when the illuminating laser reflects off the glass/water or glass/sample interface) rather than a lamp or laser. This evanescent field only penetrates 50-150 nm into the sample providing extremely high signal to noise, as those fluorophores found further than this from the interface are not excited.
Farmer, Rollason, Whitcomb, Ni, Goodliff, Lay, Birnbaumer, Heesom, Xu, Saleem, Welsh (2019) TRP6 binds and activates calpain, independent of its channel activity, and regulates podocyte cytoskeleton, cell adhesion and motility Journal of the American Society of Nephrology ASN.2018070729
Evans, Gurung, Wilkinson, Stephens, Henley (2017) The assembly, secretory pathway trafficking, and surface delivery of kainate receptors is regulated by neuronal activity. Cell Reports 19: 2613–2626.