Total internal reflection fluorescence (TIRF)
The Wolfson Bioimaging Facility provides total internal reflection fluorescence microscopy (TIRF) with its multi-laser, dual-camera Olympus/Abbelight SAFe360 system which also offers single molecule super-resolution imaging.
TIRF microscopy provides extremely high axial resolution by exciting the fluorophores with an evanescent field (caused when the illuminating laser reflects off the glass/water or glass/sample interface) rather than a lamp or laser. This evanescent field only penetrates 50-150 nm into the sample providing extremely high signal to noise, as those fluorophores found further than this from the interface are not excited.
Abbelight TIRF + SM technical specifications (PDF, 160kB)
Recent papers:
- Kuzmuk, Pranke, Rollason, Butler, Ding, Beesley, Waters, Coward, Sessions, Tuffin, Foster, Mollet, Antignac, Edelman, Welsh, Saleem. (2024) A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic nephrotic syndrome. Kidney International 105: 744-758
- Lu, Veler, Simonetti, Raj, Chou, Cross, Phillips, Ruan, Huynh, Dowsey, Ye, Murphy, Verkade, Cullen, Wülfing (2023) Five inhibitory receptors display distinct vesicular distributions in murine T cells. Cells 12(21):2558<
- Ding, Kuzmuk, Hunter, Lay, Hayes, Beesley, Rollason, Hurcombe, Barrington, Masson, Cathery, May, Tuffin, Roberts, Mollet, Chu, Mcintosh, Coward, Antignac, Nathwani, Welsh, Saleem (2023) Adeno-associated virus gene therapy prevents progression of kidney disease in genetic models of nephrotic syndrome Science Translational Medicine DOI: 10.1126/scitranslmed.abc8226