| Dual immunofluorescent labelling combined
with strong autofluorescence of internal elastic lamina (green) provides
a three-colour image of rat artery. TRITC-conjugated antibodies localise
the inwardly rectifying potassium channel (Kir2.1; red) and AMCA-conjugated
antibodies localise alpha-actinin in smooth muscle (blue). Image obtained
using a Leica TCS NT confocal microscope system with Kr/Ar and Ar-UV lasers.
Jane Hinton, Department of Pharmacology |