ARNO Translocation
|
|
Insulin stimulated translocation of a GFP-tagged
ARNO in 3T3-L1 adipocytes. The movie shows a typical adipocyte imaged by
laser-scanning confocal microscopy at 10 sec. intervals using a leica TCS
NT confocal imaging system. Insulin was added after 120 sec (INS) and wortmannin
at 250 sec. (WM). The addition of insulin causes a rapid (approx. 30 second)
translocation of GFP-ARNO to the plasma membrane where it binds PIP3.
This effect is completely reversed by the PtdIns 3-kinase inhibitor wortmannin.
This work is published in Venkateswarlu, K., Oatey, P.B., Tavaré, J.M., and Cullen, P.J. (1998) Curr. Biol. 8, 463-466 |