Dr David Matthews
Senior Lecturer in Virology
School of Cellular and Molecular Medicine,
University of Bristol, Medical Sciences Building,
Bristol, BS8 1TD
phone: +44 (0)117 33 12058 (internal 12058)
email: d.a.matthews@bristol.ac.uk
Research interests
I am primarily interested in virus host cell interactions with an emphasis on the common cold virus, adenovirus. I have been looking at how this virus interacts with the host cell using state of the art techniques including laser confocal microscopy, high throughput quantitative mass spectrometry and, most recently, Deep Sequencing (or Next Generation Sequencing) of adenovirus infected cells. In addition, I am working to develop new approaches to study how recombinant adenovirus vectors interact with the host during gene therapy treatment.
Adenovirus is often regarded as a model virus system and has been very useful in understanding not only how viruses infect and take over cells but it has also helped us to understand how cells work normally. Most recently, this virus has been developed as a gene therapy system and as a prospective cancer-killing virus. Thus, understanding how adenovirus interacts with human cells underpins the development of this virus as a new treatment for cancer and genetically inherited diseases.
Selected publications
- Emmott, E., Wise, H., Loucaides, E.M., Matthews, D.A., Digard, P. and Hiscox, J. (2010) Quantitative proteomics using SILAC coupled to LC-MS/MS reveals changes in the nucleolar proteome in influenza virus infected cells. J. Proteome Research, 9, 5335-5345.
- Hiscox, J.A., Whitehouse, A. and Matthews, D.A. (2010) Nucleolar proteomics and viral infection. Proteomics, 10, 4077-4086.
- Lam, Y.W., Evans, V.C., Heesom, K.J., Lamond, A.I. and Matthews, D.A. (2010) Proteomic analysis of the nucleolus in adenovirus infected cells. Mol. Cell. Proteomics, 9, 117-130. Highlighted in that months edition.
- Ugai, H, Wang, M, Le, L.P., Matthews, D.A., Yamamoto, M. and Curiel, D.T. (2010) In vitro dynamic visualization analysis of fluorescently labeled minor capsid protein IX and core protein V by simultaneous detection. J. Mol. Biol., 395, 55-78.
- Stow, N.D., Evans, V.C. and Matthews, D.A. (2009). Upstream Binding Factor is sequestered into Herpes Simplex Virus type 1 replication compartments. J. Gen. Virol. 90, 69-73.
- Hindley, C.E., Davidson, A.D. and Matthews, D.A. (2007) The relationship between adenovirus DNA replication proteins and nucleolar proteins B23.1 and B23.2. J. Gen. Virol. 88, 3244-8.
- Matthews, D.A. (2007). Study of nucleolar localization of adenovirus core proteins. Methods Mol. Med. 131, 73-82.
- Hindley, C.E., Lawrence, F.J. and Matthews, D.A. (2007) A role for transportin in the nuclear import of adenovirus core proteins and DNA. Traffic. 8,1313-22.