MULTIBAC
MultiBac is an advanced baculovirus/insect cell expression platform specifically tailored for the production of eukaryotic multiprotein complexes and complex biologics including therapeutic proteins. MultiBac is installed in the Berger laboratory at University of Bristol, School of Biochemistry. MultiBac can be accessed by researchers from academia and industry at Bristol’s EEF (contact imre.berger@bristol.ac.uk for details).
MultiBac utilises an optimised baculovirus that has been engineered in the Berger lab for high-quality protein production. A fluorescent marker has been integrated into the viral backbone to enable virus performance tracking and production of complex biologics. This virus is accessed by multigene constructs containing the DNA encoding for the protein complex produced. This multigene construct is generated from small synthetic precursor plasmid modules into which the genes of interest are inserted. Interfaced with the proprietary ACEMBL pipeline the MultiBac platform can be used for biologics production in high-throughput mode.
MultiBac was awarded prestigious prizes including the Swiss Technology Award in 2007, and continues to attract competitive funding from the European Commission since FP6 and throughout FP7. The MultiBac platform has been accessed by many scientists from Europe and world-wide for intensive training and for protein production. It is successfully integrated in numerous co-developments, R&D and discovery programmes, in academia and industry.
For in silico generating Donor-Acceptor fusion constructs you can use Cre-ACEMBLER.