Correlative light electron microscopy (CLEM)

The Wolfson Bioimaging Facility is specifically equipped to combine light and electron microscopy and to undertake CLEM experiments. By utilising the advantages of fluorescence microscopy (e.g. live cell imaging) with the higher resolution and contextual information which EM provides, CLEM can deliver considerably more information than either modality alone.

 

 

Recent papers including CLEM data:

• Latta, Trinca, Robertson, Lau, Barkaway, Rolas, Golding, Imbert, Wang, Bader, Hill, Hodgson, Yang, Ivetic, Walzog, Lewis, Voisin, Martin, Nourshargh, Weavers (2026) Orphan GPR84 facilitates uropod de-adhesion at multiple steps during leukocyte extravasation. EMBO Rep. doi: 10.1038/s44319-026-00923-0
• Glover, Wiseman, Dugdale, Humphery, Sueiro Ballesteros, Hodgson, Wilkinson, Collinson (2026)    Intercellular mitochondrial transfer and trans-mitophagy in response to protein import dysfunction Journal of Cell Biology. doi: 10.1083/jcb.202511211
• Hilditch, Romanyuk, Hodgson, Mantell, Neal, Verkade, Obexer, Serpell, McManus, Woolfson (2024) Maturation and conformational switching of a de novo designed phase-separating polypeptide. J. Am. Chem. Soc. doi: 10.1021/jacs.4c00256 
• Pope, Tanner, Masia, Payne, Arkill, Mantell, Langbein, Borri, Verkade (2023) Correlative light-electron microscopy using small gold nanoparticles as single probes. Light Sci Appl. 12(1)80
• Qiu, Buffonge, Ramnath, Jenner, Fawaz, Arkill, Neal, Verkade, White, Hezzell, Salmon, Suleiman, Welsh, Foster, Madeddu, Satchell (2022) Endothelial glycocalyx is damaged in diabetic cardiomyopathy: angiopoietin 1 restores glycocalyx and improves diastolic function in mice. Diabetologia. 2022 May;65(5):879-894.
• Baines, Yoshioka, Takuwa, Lane (2022) The ATG5 interactome links clathrin-mediated vesicular trafficking with the autophagosome assembly machinery. Autophagy Reports 1: 88-118