
Professor Jon Lane
B.Sc.(Soton.), Ph.D.(Exon.)
Expertise
My lab work studies autophagy and other cellular responses to stress. We use transformed human cells and human iPSC-derived neurons and glia to understand how autophagy shapes neuronal responses to stress Parkinson's.
Current positions
Professor of Cell Biology
School of Biochemistry
Contact
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Biography
I graduated with a BSc in Biology from Southampton University, and then studies for a PhD in Exeter on the cell cycle regulation of microtubule dynamics. This led to PostDoctoral work in Manchester on developmental microtubule motor control, and organelle disruption and altered protein trafficking in apoptosis. A Wellcome Trust Career Development Fellowship brought me to Bristol, where I have continued researching cellular responses to stress, with a focus on membrane trafficking and dynamics.
Over recent years, my group has concentrated on the process of autophagy - a crucial cellular stress response pathway that plays important roles in a variety of human diseases. We study how autophagy influences neuronal resilience in Parkinson's, using human induced pluripotent stem cells (hiPSCs) from which we grow human neurons and glia in the lab.
Research interests
My lab is interested in autophagy - the regulated recycling of cytoplasmic material through delivery and degradation in the lysosome.
Please visit the Lab website https://thelanelab.blogs.bristol.ac.uk/
Follow Jon on Twitter @Jon_D_Lane
Autophagy (macroautophagy) is characterised by the formation of double membrane-bound organelles that sequester regions of cytoplasm including misfolded protein aggregates and organelles.The autophagosome membrane is decorated with a protein called Atg8 (commonly known as LC3), which plays roles in autophagosome assembly and cargo selection.
We use live-cell imaging as well as fixed cell microscopy (including electron microscopy) to explore how and where autophagosomes are assembled. Through the application of cell-lines expressing GFP-tagged autophagy proteins (such as Atg5; Atg14; Atg16L; DFCP1), we can determine how the sequential recruitment of autophagy factors influences autophagosome assembly.
In our studies we used various human and mouse cell culture lines, primary human erythroid precursors and induced pluripotent stem cells from human patients. The latter we differentiate into specific neuronal lineages to understand how autophagy is regulated in neurons for research into the causes of neurodegenerative diseases (Parkinson’s disease; Alzheimer’s disease).
Projects and supervisions
Research projects
Autophagy and skelelal ageing
Principal Investigator
Managing organisational unit
School of BiochemistryDates
01/12/2023 to 30/11/2026
BBSRC ALERT 21 LCM-MM: Bringing Laser Capture Microscopy Technology to the University of Bristol
Principal Investigator
Managing organisational unit
School of BiochemistryDates
01/03/2022 to 28/02/2023
Autophagy Transcriptional Crosstalk: The LMX1A/LMX1B Paradigm
Principal Investigator
Managing organisational unit
School of BiochemistryDates
13/10/2020 to 12/10/2023
Autophagy Transcriptional Crosstalk: The LMX1A/LMX1B Paradigm
Principal Investigator
Managing organisational unit
Bristol Medical School (THS)Dates
01/10/2020 to 30/09/2023
Autophagy transcriptional crosstalk: the LMX1A/LMX1B paradigm
Principal Investigator
Managing organisational unit
School of BiochemistryDates
01/07/2014
Thesis supervisions
The Engineering and Evaluation of Genome Editing Tools in Mitochondria
Supervisors
Proteomics-Based Analysis of The Coordination of Membrane Trafficking Events by The ATG12-ATG5 Autophagy Complex
Supervisors
Glial autophagy capability and the control of neuroinflammatory signaling in Parkinson’s disease.
Supervisors
Investigating the role of autophagy in musculoskeletal development and homeostasis using zebrafish
Supervisors
LC3-associated phagocytosis and its potential neuroinflammatory role in Parkinson’s Disease
Supervisors
Defining the relationship between the mTORC1-autophagy pathway and Focal Adhesions
Supervisors
Toward a thorough mechanistic understanding of PINK1 mitochondrial import
Supervisors
Reciprocal Autophagy Control by the LIM Homeodomain Transcription Factors LMX1A and LMX1B Safeguards Human Midbrain Dopaminergic Neurons
Supervisors
RhoJ regulates Tie2 trafficking and signalling in endothelial cells
Supervisors
Publications
Recent publications
01/12/2024Reactive astrocytes generated from human iPSC are pro-inflammatory and display altered metabolism
Experimental Neurology
International consensus guidelines for the definition, detection, and interpretation of autophagy-dependent ferroptosis
Autophagy
ATG8-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience
Journal of Cell Biology
ATG8 proteins are co-factors for human dopaminergic neuronal transcriptional control: implications for neuronal resilience in Parkinson disease
Autophagy
Human stem cell-derived ventral midbrain astrocytes exhibit a region-specific secretory profile
Brain Communications
Teaching
I teach on all years of the Biochemistry BSc/MSci programmes, covering mitosis and the cell cycle, apoptosis, and autophagy in health and disease. I also contribute to teachng on MSc and PhD programmes, including through lectures, Journal clubs, tutorials, and workshops.