Dr Jon Lane
B.Sc.(Soton.), Ph.D.(Exon.)
Expertise
My lab work studies autophagy and other cellular responses to stress. We use transformed human cells and human iPSC-derived neurons and glia to understand how autophagy shapes neuronal responses to stress Parkinson's.
Current positions
Reader in Cell Biology
School of Biochemistry
Contact
Media contact
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Biography
I graduated with a BSc in Biology from Southampton University, and then studies for a PhD in Exeter on the cell cycle regulation of microtubule dynamics. This led to PostDoctoral work in Manchester on developmental microtubule motor control, and organelle disruption and altered protein trafficking in apoptosis. A Wellcome Trust Career Development Fellowship brought me to Bristol, where I have continued researching cellular responses to stress, with a focus on membrane trafficking and dynamics.
Over recent years, my group has concentrated on the process of autophagy - a crucial cellular stress response pathway that plays important roles in a variety of human diseases. We study how autophagy influences neuronal resilience in Parkinson's, using human induced pluripotent stem cells (hiPSCs) from which we grow human neurons and glia in the lab.
Research interests
My lab is interested in autophagy - the regulated recycling of cytoplasmic material through delivery and degradation in the lysosome.
Please visit the Lab website https://thelanelab.blogs.bristol.ac.uk/
Follow Jon on Twitter @Jon_D_Lane
Autophagy (macroautophagy) is characterised by the formation of double membrane-bound organelles that sequester regions of cytoplasm including misfolded protein aggregates and organelles.The autophagosome membrane is decorated with a protein known as Atg8 (commonly known as LC3), which plays roles in autophagosome assembly and cargo selection.
We use live-cell imaging as well as fixed cell microscopy (including electron microscopy) to explore how and where autophagosomes are assembled. Through the application of cell-lines expressing GFP-tagged autophagy proteins (such as Atg5; Atg14; Atg16L; DFCP1), we can determine how the sequential recruitment of autophagy factors influences autophagosome assembly.
In our studies we used various human and mouse cell culture lines, primary human erythroid precursors and induced pluripotent stem cells from human patients. The latter we differentiate into specific neural lineages to understand how autophagy is regulated in neurons for research into the causes of neurodegenerative diseases (Parkinson’s disease; Alzheimer’s disease).
Positions
University of Bristol positions
Reader in Cell Biology
School of Biochemistry
Projects and supervisions
Research projects
Autophagy transcriptional crosstalk: the LMX1A/LMX1B paradigm
Principal Investigator
Managing organisational unit
School of BiochemistryDates
01/07/2014
Autophagy Transcriptional Crosstalk: The LMX1A/LMX1B Paradigm
Principal Investigator
Managing organisational unit
School of BiochemistryDates
13/10/2020 to 12/10/2023
Remodelling the isolation membrane: roles of sorting nexins during autophagy
Principal Investigator
Managing organisational unit
School of BiochemistryDates
01/01/2012 to 01/01/2015
Thesis supervisions
The Engineering and Evaluation of Genome Editing Tools in Mitochondria
Supervisors
Reciprocal Autophagy Control by the LIM Homeodomain Transcription Factors LMX1A and LMX1B Safeguards Human Midbrain Dopaminergic Neurons
Supervisors
Proteomics-Based Analysis of The Coordination of Membrane Trafficking Events by The ATG12-ATG5 Autophagy Complex
Supervisors
Investigation into the Effect of Protein Disulphide Isomerase A3 on the Prom-Metastatic Phenotype of Breast Cancer Cells
Supervisors
Publications
Recent publications
24/09/2020Efficient and scalable generation of human midbrain astrocytes from hiPSCs
Journal of Visualized Experiments
Mitochondrial import, health and mtDNA copy number variability using Type II and Type V CRISPR effectors
Journal of Cell Science
A heterodimeric SNX4:SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly
Journal of Cell Science
A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons
Autophagy
LIR-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience
Journal of Cell Biology
Teaching
I teach on all years of the Biochemistry BSc/MSci programmes, covering mitosis and the cell cycle, apoptosis, and autophagy in health and disease. I also contribute to teachng on MSc and PhD programmes, including through lectures, Journal clubs, tutorials, and workshops.