Yolanda Schaerli, Université de Lausanne

10 February 2021, 1.00 PM - 10 February 2021, 2.00 PM

Zoom Webinar link: https://bristol-ac-uk.zoom.us/j/99672940246 Slido link: #25482

Multistable and dynamic CRISPRi-based synthetic circuits

Prof. Yolanda Schaerli
Université de Lausanne
Slido meeting number: #25482

Gene expression control based on CRISPRi (clustered regularly interspaced short palindromic repeats interference) has emerged as a powerful tool for creating synthetic gene circuits, both in prokaryotes and in eukaryotes; yet, its lack of cooperativity has been pointed out as a potential obstacle for dynamic or multistable synthetic circuit construction. In my talk I will present how we used CRISPRi to build a synthetic oscillator (“CRISPRlator”), bistable network (toggle switch) and a collection of stripe pattern-forming incoherent feed-forward loops in Escherichia coli. Our work demonstrates the wide applicability of CRISPRi in synthetic circuits and paves the way for future efforts towards engineering more complex synthetic networks.


Contact information

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