Yolanda Schaerli, Université de Lausanne
Zoom Webinar link: https://bristol-ac-uk.zoom.us/j/99672940246 Slido link: #25482
Multistable and dynamic CRISPRi-based synthetic circuits
Gene expression control based on CRISPRi (clustered regularly interspaced short palindromic repeats interference) has emerged as a powerful tool for creating synthetic gene circuits, both in prokaryotes and in eukaryotes; yet, its lack of cooperativity has been pointed out as a potential obstacle for dynamic or multistable synthetic circuit construction. In my talk I will present how we used CRISPRi to build a synthetic oscillator (“CRISPRlator”), bistable network (toggle switch) and a collection of stripe pattern-forming incoherent feed-forward loops in Escherichia coli. Our work demonstrates the wide applicability of CRISPRi in synthetic circuits and paves the way for future efforts towards engineering more complex synthetic networks.
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