Unit name | Recombinant DNA Technology |
---|---|
Unit code | MOLG22100 |
Credit points | 20 |
Level of study | I/5 |
Teaching block(s) |
Teaching Block 1 (weeks 1 - 12) |
Unit director | Professor. Szczelkun |
Open unit status | Not open |
Pre-requisites |
None |
Co-requisites |
None |
School/department | School of Biochemistry |
Faculty | Faculty of Life Sciences |
This unit examines the methodology and applications of recombinant DNA technology. Topics covered include: genome organisation; procedures for cloning and analysing genes; methods for analysing gene expression; the creation and use of transgenic microorganisms, plants and animals; gene editing; synthetic DNA and genomes; protein engineering; bioinformatics; microarray technology. 30 lectures are supplemented with five practical sessions and three tutorials. Assessment is by sessional examination (80%), assessed exercises associated with the tutorials (10%) and laboratory reports (10%). Some prior knowledge of molecular biology (eg equivalent to Biochemistry: Cellular Composition) is recommended.
The main aim of this Unit is to provide students with a good understanding of the techniques used to clone, analyse and modify genetic material in microorganisms, plants and animals. Further aims are to provide them with practical experience in some of these techniques, and to improve their ability to critically assess experimental data.
Learning outcomes: knowledge and understanding
Upon completion of this course students should understand and be able to explain:
Learning outcomes: skills
Upon completion of this course students should have acquired the skills necessary to:
Lectures, tutorials and practicals
Continual assessment:
20% of the total marks for the unit are awarded on the basis of assessment exercises undertaken during the course. Thus 5% of the final assessment mark is awarded for a data-handling exercise, 5% is awarded for the combined mark for two assessed essays, and 10% is awarded for the combined mark for practical write-ups.
Sessional exam:
80% of the total marks for the unit are awarded for performance in the unit examination in January, which will consist of one 2.5-hour paper. The paper is split into three sections: the first section (40% of the exam marks) will comprise 50 multiple choice questions (MCQs); the second section (30% of the exam marks) will contain three essay questions of which candidates will be asked to answer one; the third section (30% of the exam marks) will contain three essay questions of which candidates will be asked to answer one. two, each carrying equal marks.