University of Bristol
Proteomics Facility
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Related Literature
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Related Literature

Technical Information

ThermoFisher Scientific Mass Spec. Handbook

http://www.piercenet.com/Files/1601927_MSHBv2_Intl.pdf

TMT approach

http://www.piercenet.com/previews/Vol12Iss03/1601715_PreviewsV12N3_pg25-27.pdf

2D Fluorescence Difference Gel Electrophoresis (2D-DIGE)

User Manual. Amersham Biosciences
http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/literature_intro
Select main product area Proteomics and sub-area Fluorescence difference 2D gel electrophoresis.

Recent Publications

  1. Jefferies R, Morganb E, Shawa S and Heesom K. (2011) Molecular and Biochemical Parasitology (EPub ahead of print). Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry.
  2. Barker EN, Darby AC, Helps CR, Peters IR, Heesom KJ, Arthur CJ, Crossett B, Hughes MA, Radford AD, and Tasker, S. (2011) Vet Res 42, 83-91. Molecular characterisation of the uncultivatable hemotropic bacterium Mycoplasma haemofeils.
  3. Vincent EE, Elder DJ, Phillips L, Heesom KJ, Pawade J, Luckett M, Sohail M, May MT, Hetzel MR and Tavaré JM. (2011) Anticancer Res. 31, 1577-1582. Overexpression of the TXNDC5 protein in Non-small cell ling carcinoma.
  4. Barker EN, Helps CR, Heesom KJ, Arthur CJ, Peters IR, Hofmann-Lehmann R. and Tasker S. (2010) Clinical and Vaccine Immunology 17, 1926-1932. Detection of humoral response using a recombinant heat shock protein 70, DnaK, of Mycoplasma haemofelis in experimentally and naturally hemoplasma-infected cats.
  5. Bergmeier L, Heesom KJ, Babaahmady K, Kelly CG, Pido-Lopez J. and Lehner T. (2010) Immunology 129, 506-515. Cytoskeletal proteins bound to hsp70 may elicit resistance to SIV infection of CD4+ T cells.
  6. Lam YW, Evans VC, Heesom KJ, Lamond AI and Matthews DA. (2010). Molecular and Cellular Proteomics 9, 117-130. Proteomic Analysis of the nucleolus in adenovirus infected cells.
  7. Camats M, Kokolo M, Heesom KJ, Ladomery M and Bach-Elias M. (2009). PLoS One 4, e8518. P19 H-Ras Induces G1/S Phase Delay Maintaining Cells in a Reversible Quiescence State.
  8. Kemp K, Hares K, Mallam E, Heesom KJ Scolding N and Wilkins A. (2009). J. Neurochem 114, 1569-1580. Mesenchymal stem cell-secreted superoxide dismutase promotes cerebellar neuronel survival.
  9. Bhahra G, Sood A, Fisher B, Cartwright L, Saunders M, Evans WH, Surpenant A, Lopez-Castejon G, Mann S, Davis SA, Hails LA, Ingham I, Verkade P, Lane J, Heesom K, Newson R and Case CP (2009) Nature Nanotechnology 4, 876-883. Nanoparticles can cause DNA damage across a cellular barrier.
  10. Richardson, B.M., Heesom, K.J., Parsons, S.F., Anstee, D.J and Frayne, J. (2009) Analysis of the differential proteome of human erythroblasts during in vitro erthyropoiesis by 2D DIGE. Proteomics - Clinical Applications 3, 1-12.
  11. Russell, C.L., Heesom, K.J., Arthur, C.J., Helps, C.R., Mellor, P.S., Day, M.J., Torsteinsdottir, S., Björnsdóttir, T. S. and Wilson A.D. (2009) Identification and isolation of cDNA clones encoding the abundant secreted proteins in the saliva proteome of Culicoides nubeculosus. J insect molecular Biology 18, 383-393.
  12. Van Helmond, Z., Heesom, K.J. and Love, S. (2009) Characterisation of two antibodies to oligomeric Aß and their use in ELISAs on human brain tissue homogenates. J. Neuroscience Methods 176, 206-212.
  13. Clarke, S.J., Khaliulin, I., Das, M., Parker, J.E., Heesom, K.J. and Halestrap, A.P. (2008) Inhibition of mitochondrial permeability transition pore opening by ischemic preconditioning is mediated by reduction of oxidative stress rather than mitochondrial protein phosphorylation. Circulation Research 102, 1082-1090.
  14. Wilson, A.D., Heesom, K.J., Mawby, W.J., Mellor, P.S. and Russell, C.L. (2008). Identification of abundant proteins and potential allergens in Culicoides nubeculosus salivary glands. Vet. Immunol. Immunopathol. 102, 94-103.
  15. Griffiths, R.E., Heesom, K.J. and Anstee, D.J. (2007). Normal Prion Protein Trafficking in Cultured Human Erythroblasts. Blood 110, 4518-4525.
  16. Gouraud, S.S., Yao, S.T., Heesom, K.J., Paton, J.F.R and Murphy, D. (2007) 14-3-3 proteins within the hypothalamo-neurohypophyseal system of the osmotically stressed rat: transcriptomic and proteomic studies. J. Endocrinology 19, 913-922.
  17. Gouraud, S.S., Heesom, K.J., Yao, S.T., Qiu, J., Paton, J.F.R. and Murphy, D. (2007) Dehydration-induced proteome changes in the rat hypothalamo-neurohypophyseal system. Endocrinology 148, 3041-3052.
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