Equipment
Liquid Chromatography Mass Spectrometry
Orbitrap Fusion Lumos Mass Spectrometer (Thermo Scientific)
Operated with up-stream Ultimate 3000 nano-LC system.
Thermo Scientific's most advanced mass spectrometry system.
Ultra-high resolution and accurate mass instrument used for:
- Protein identification from highly complex samples
- SILAC and phospho-SILAC
- TMT and phospho-TMT (using SPS-MS3 analysis)
Orbitrap Fusion Tribrid Mass Spectrometer with ETD (Thermo Scientific)
Operated with up-stream Ultimate 3000 nano-LC system.
Ultra-high resolution and accurate mass instrument used for:
- Protein identification from highly complex samples
- SILAC and phospho-SILAC
- TMT and phospho-TMT (using SPS-MS3 analysis)
LTQ-Orbitrap Velos Mass Spectrometer with ETD (Thermo Scientific)
Operated with up-stream Ultimate 3000 nano-LC system.
Ultra-high resolution and accurate mass instrument used for:
- Protein interaction studies (AP-MS, IPs, etc.)
- Protein identification from less complex samples
MALDI-Mass spectrometry
Bruker Daltonics UltrafleXtreme 2 (housed in the School of Chemistry Mass Spectrometry Facility)
High resolution and accurate mass instrument used for:
- Protein identification from single gel bands
Automated Proteolytic Digestion
DigestPro MSi Robot (Intavis Bioanalytical Instruments)
Automates proteolytic digestion to minimise sample handling. 96-well plate format allows the digestion of multiple samples in a single run.
Liquid Chromatography
Ultimate 3000 Bio-RS HPLC system (Thermo Scientific)
Used for the fractionation of peptide mixtures using basic RP chromatography, SAX chromatography or SCX chromatography.