The BioSuite is a custom-built facility that supports microbial cell culture and protein production
The BioSuite will allow you to design experiments that include high throughput molecular biology, protein production, fermentation, assays, library generation and structural biology, especially the preparation of crystallisation plates. The facility is a containment level 1 laboratory and is mainly focussed on bacterial and yeast work.
Access charges are available here for University of Bristol users.
External users, including University of Bristol spin-outs should contact the BioSuite Manager, Dr Peter Wilson, for pricing.
Tecan Freedom EVO 150
Liquid handling robot with integrated plate reader for general liquid handling tasks and automation of assays.
Tecan Freedom EVO 200
Liquid handling robot for molecular biology and protein production. This system allows for multi-parallel cloning and protein expression screening in a high-throughput manner. The robot facilitates PCR amplification, product purification, ligation, DNA extraction, plasmid transformation and colony picking along with microbial cell culture in a 96 well plate format. This allows screening for protein expression and solubility and for culturing strains for metabolite extraction.
GE AKTA explorer
Fast protein liquid chromatography system for protein purification.
Formulatrix RI-1000 crystallisation hotels
One at 4°C and other at 20°C, for storage and monitoring of crystal plates.
Crystallography dispensing robots
For sitting and hanging drop vapour diffusion experiments on the nanolitre to microlitre scale, supporting microseeding, additive screening and co-crystallisations: TTP Labtech Mosquito Xtal 3 with humidity chamber; Art Robbins Phoenix; Art Robbins Gryphon LCP.
specialised for small molecule and limited peptide analysis: Waters Alliance HPLC and QDa; Waters Acquity UPLC and Xevo G2-XS QTof.
- New Brunswick Innova 44R incubators x 3
- Stuart SI505 plate incubators x2
- Geneflow Sensoquest basic thermocycler
- Eppendorf 5810 plate centrifuge