Publications

The following research publications relate to work conducted at TBRC.

• Development and translation of thiometallate sulfide donors using a porcine model of coronary occlusion and reperfusion. Redox Biology, Vol 23, July 2024, 103167. Thomas W. Johnson, James Holt, Anna Kleyman, Shengyu Zhou, Eva Sammut, Domenico Bruno,Charlotte Gaupp, Giacomo Stanzani, John Martin, Pietro Arina, Julia Deutsch, Raimondo Ascione, Mervyn Singer, Alex Dyson (2024).
  
  
• In situ monolayer patch clamp of acutely stimulated human iPSC-derived cardiomyocytes promotes consistent electrophysiological responses to SK channel inhibition. Scientific Reports, [online] 14(1), p.3185.Butler, A.S., Ascione, R., Marrion, N.V., Harmer, S.C. and Hancox, J.C. (2024). https://doi.org/10.1038/s41598-024-53571-6
  
  
• A quantitative evaluation of aerosol generation during cardiopulmonary resuscitation. Anaesthesia. Shrimpton, A., Brown, V.J., Vassallo, J., Nolan, J.P., Soar, J., Hamilton, F., Cook, T., Bzdek, B.R., Reid, J.P., Makepeace, C., Deutsch, J., Ascione, R., Brown, J., Benger, J. and Pickering, A.E. (2023). https://doi.org/10.1111/anae.16162
  
  
• Biological Scaffolds for Congenital Heart Disease. Bioengineering, 10(1), 1-27. Article 57.  Harris, A. G. , Salih, T. K. O., Ghorbel, M., Caputo, M., Biglino, G., & Carrabba, M. (2023). https://doi.org/10.3390/bioengineering10010057
  
  
• Development of a Novel Hierarchically Biofabricated Blood Vessel Mimic Decorated with Three Vascular Cell Populations for the Reconstruction of Small-Diameter Arteries. Advanced Functional Materials. Michele Carrabba, Marco Fagnano, Mohamed T Ghorbel, Filippo Rapetto, Bo Su, Carmelo De Maria, Giovanni Vozzi, Giovanni Biglino, Adam W. Perriman, Massimo Caputo, Paolo Madeddu. (2023) https://doi.org/10.1002/adfm.202300621
  
  
• The effect of cardioplegic supplementation with sildenafil on cardiac energetics in a piglet model of cardiopulmonary bypass and cardioplegic arrest with warm or cold cardioplegia. In: Frontiers in Cardiovascular Medicine. 10, p. 1194645Skeffington, K. L., Mohamed Ahmed, E., Rapetto, F., Chanoit, G., Bond, A. R., Vardeu, A., Ghorbel, M. T., Suleiman, M-S. & Caputo, M., 2023. https://doi.org/10.3389/fcvm.2023.1194645
  
  
• A novel small diameter nanotextile arterial graft is associated with surgical feasibility and safety and increased transmural endothelial ingrowth in pig. Journal of Nanobiotechnology, 20(1), 1-13. [71].Josef, J., Bruno, V. D., Sulaiman, N., Ward, A., Johnson, T. W., Baby, H. M., Nair, S. V., Menon, D., George, S. J., & Ascione, R. (2022). https://doi.org/10.1186/s12951-022-01268-1
  
  
• Decellularised Human Saphenous Veins. International Journal of Molecular Sciences, 23(12), 1-22. [6633]Bond, A. R., Bruno, V. D., Johnson, J. L., George, S. J., & Ascione, R. (2022). https://doi.org/10.3390/ijms23126633
  
  
• Development and Preliminary Testing of Porcine Blood Derived Endothelial-like Cells for Vascular Tissue Engineering Applications: Protocol Optimisation and Seeding of Decellularised Human Saphenous Veins. International Journal of Molecular Sciences, 23(12), 1-22. [6633]Bond, A. R., Bruno, V. D., Johnson, J. L., George, S. J., & Ascione, R. (2022). https://doi.org/10.3390/ijms23126633
  
  
• Development and Preliminary Testing of Porcine BloodDerived Endothelial-like Cells for Vascular Tissue Engineering Applications: Protocol Optimisation and Seeding of Decellularised Human Saphenous Veins. International Journal of Molecular Sciences, 23(12), 1-22. Article 6633. Bond, A. R. , Bruno, V. D., Johnson, J. L., George, S. J., & Ascione, R.(2022). https://doi.org/10.3390/ijms23126633
  
  
• Development of a cardiovascular magnetic resonance compatible large animal isolated heart model for direct comparison of beating and arrested hearts. NMR in Biomedicine, 35(7), [e4692]. Scott, A. D. , Bruno, V. D., Doorly, D. J., Pennell, D. J., Ascione, R., de Silva, R., Firmin, D. N., Rose, J. N., Sarathchandra, P., McCarthy, K. P., Roehl, M., Nialles-Vallespin, S., Ferreira, P. F., Chowdhury, R. A., Begum, L., Pardoe, B., Gorodezky, M., Khalique, Z., & Jackson, T. (2022). https://doi.org/10.1002/nbm.4692
  
  
• Improved Ca2+ release synchrony following selective modification of Itof and phase 1 repolarization in normal and failing ventricular myocytes. Journal of Molecular and Cellular Cardiology, 172, pp.52–62.Fowler, E.D., Wang, N., Hezzell, M.J., Chanoit, G., Hancox, J.C. and Cannell, M.B. (2022). doi:https://doi.org/10.1016/j.yjmcc.2022.07.009.
  
  
• Inducing Ito,f and phase 1 repolarization of the cardiac action potential with a Kv4.3/KChIP2.1 bicistronic transgene. Journal of Molecular and Cellular Cardiology, [online] 164, pp.29–41. Wang, N., Dries, E., Fowler, E.D., Harmer, S.C., Hancox, J.C. and Cannell, M.B. (2022). doi:https://doi.org/10.1016/j.yjmcc.2021.11.004.
  
  
• Signaling and structures underpinning conducted vasodilation in human and porcine intramyocardial coronary arteries. Frontiers in Cardiovascular Medicine, 9, 1-39. [980628]. Dora, K. A., Lin, J., Borysova, L., Beleznai, T., Taggart, M. , Ascione, R., & Garland, C. J. (2022). https://doi.org/10.3389/fcvm.2022.980628
  
  
• Acute regional changes in myocardial strain may predict ventricular remodelling after myocardial infarction in a large animal model. Nature Sci Rep 11, 18322 (2021).Mansell, D.S., Bruno, V.D., Sammut, E. et al. https://doi.org/10.1038/s41598-021-97834-y.
  
  
• Acute regional changes in myocardial strain may predict ventricular remodelling after myocardial infarction in a large animal model. Scientific Reports, 11(1), Article 18322. Mansell, DS. , Bruno, V. D., Sammut, E., Chiribiri, A., Johnson, T. W., Khaliulin, I., Baz Lopez, D., Gill, HS., Fraser, KH., Murphy, M., Krieg, T., Suleiman, M-S., George, S. J., Ascione, R., & Cookson, AN. (2021). https://doi.org/10.1038/s41598-021-97834-y
  
  
• Effective decellularisation of human saphenous veins for biocompatible arterial tissue engineering applications: Bench optimisation and feasibility in vivo testing. Journal of Tissue Engineering, 12, [2041731420987529].Sulaiman, N. S., Bond, A. R., Bruno, V. D., Joseph, J., Johnson, J. L., Suleiman, M-S., George, S. J., & Ascione, R. (2021). https://doi.org/10.1177/2041731420987529
  
  
• High spatial and temporal resolution Ca2+ imaging of myocardial strips from human, pig and rat. Nature Protocols, 16(10), 4650-4675. Borysova, L., Hanson Ng, Y. Y., Wragg, E. S., Wallis, L. E., Fay, E., Ascione, R., & Dora, K. A. (2021). https://doi.org/10.1038/s41596- 021-00590-6
  
  
• Human coronary microvascular contractile dysfunction associates with viable synthetic smooth muscle cells. Cardiovascular Research, [cvab218]. Dora, K. A., Borysova, L., Ye, X., Powell, C., Beleznai, T. Z., Stanley, C. P., Bruno, V. D., Starborg, T., Johnson, E., Pielach, A., Taggart, M., Smart, N., & Ascione, R. (2021). https://doi.org/10.1093/cvr/cvab218
  
  
• Reconstruction of the swine pulmonary artery using a graft engineered with syngeneic cardiac pericytes. Frontiers in Bioengineering and Biotechnology, 9, Article 715717. Alvino, V. V.Thomas, A.C.Ghorbel, M.Rapetto, F.Narayan, S.Kilcooley, M.Iacobazzi, D.Carrabba, M.Fagnano, M.Cathery, W.R.Avolio, E., Caputo, M, & Madeddu, P. R. (2021). https://doi.org/10.3389/fbioe.2021.715717
  
  
• Arrhythmogenic late Ca2+ sparks in failing heart cells and their control by action potential configuration. Proceedings of the National Academy of Sciences, 117(5), pp.2687–2692. Fowler, E.D., Wang, N., Hezzell, M., Chanoit, G., Hancox, J.C. and Cannell, M.B. (2020). doi:https://doi.org/10.1073/pnas.1918649117.
  
  
• Comparison of the within-reader and inter-vendor agreement of left ventricular circumferential strains and volume indices derived from cardiovascular magnetic resonance imaging. PLoS ONE, 15(12), e0242908. [e0242908].Mansell, D. S., Frank, E. G., Kelly, N. S., Agostinho-Hernandez, B., Fletcher, J., Bruno, V. D., Sammut, E., Chiribiri, A., Johnson, T., Ascione, R., Bartlett, J. W., Gill, H. S., Fraser, K. H., & Cookson, A. N. (2020) https://doi.org/10.1371/journal.pone.0242908
  
  
• Mechanism of succinate efflux upon reperfusion of the ischemic heart. Cardiovascular Research, [cvaa148]. Prag, H. A., Gruszczyk, A. V., Huang, M. M., Beach, T. E., Young, T., Tronci, L., Nikitopoulou, E., Mulvey, J. F., Ascione, R., Hadjihambi, A., Shattock, M. J., Pellerin, L., Saeb-Parsy, K., Frezza, C., James, A. M., Krieg, T., Murphy, M. P., & Aksentijević, D. (2020). https://doi.org/10.1093/cvr/cvaa148
  
  
• Safety and effectiveness of a new vascular conduit engineered with syngeneic cardiac pericytes for reconstruction the pulmonary artery in piglets. Journal of the American Heart Association. Valeria V Alvino, Anita C Thomas, Mohamed Ghorbel, Filippo Rapetto, Srinivas Narayan, Michael Kilcooley, Dominga Iacobazzi, Michele Carrabba, Marco Fagnano, William R Cathery, Elisa Avolio, Massimo Caputo, Paolo R Madeddu (2020)  **Safety and effectiveness of a new vascular conduit engineered with syngeneic cardiac pericytes for reconstruction the pulmonary artery in piglets — University of Bristol

• Design, Development, Testing at ISO standards and in-vivo feasibility study of a novel Polymeric Heart Valve Prosthesis. Joanna R. Stasiak, Marta Serrani, Eugenia Biral, James Taylor, Francesco De Gaetano, Maria Laura Costantino, Vito D Bruno, Saadeh Suleiman, Raimondo Ascione, Geoff D. Moggridge (GD Moggridge and R Ascione are co-corresponding and co-senior authors). Biomaterials Science, 2020 (Accepted) (Predicted REF score 4*).  This important paper has generated huge international media coverage (>30,000 views) and could become an impact case for the university in the future.
  
  
• Mechanism of succinate efflux upon reperfusion of the ischemic heart. Hiran A. Prag1,2†, Anja V. Gruszczyk1,3†, Margaret M. Huang3, Timothy E. Beach3, Timothy Young2,4, Laura Tronci4, Efterpi Nikitopoulou4, Raimondo Ascione5, Anna Hadjihambi6, Michael J. Shattock7, Luc Pellerin6,8,9, Kourosh Saeb-Parsy3, Christian Frezza4, Andrew M. James1, Thomas Krieg2, Michael P. Murphy1, 2, and Dunja Aksentijević7,10* Cardiovascular Research 2020 (in press). (Predicted REF score 4*)
  
  
• Heterogeneity of T-Tubules in Pig Hearts HC Gadeberg, RC Bond, CHT Kong, GP Chanoit, R Ascione, MB Cannell, AF James. PloS one 11 (6), e0156862. (possible Ref score 2*).
  
  
• In Vitro and In Vivo Preclinical Testing of Pericyte-Engineered Grafts for the Correction of Congenital Heart Defects. Alvino VV, Kilcooley M, Thomas AC, Carrabba M, Fagnano M, Cathery W, Avolio E, Iacobazzi D, Ghorbel M, Caputo M, Madeddu P. J Am Heart Assoc. 2020 Feb 18;9(4):e014214. doi:10.1161/JAHA.119.014214. Epub 2020 Feb 11. (Predicted REF score 3*)
  
  
• Wharton’s Jelly-Mesenchymal Stem Cells-engineered conduit for paediatric translation in Heart Defect. Tissue Eng Part A.  Iacobazzi D, Rapetto F, Albertario A, Swim MM, Narayan S, Skeffington K, Salih T, Alvino VV, Madeddu P, Ghorbel MT, Caputo M. 2020 Jun 22. doi: 10.1089/ten.TEA.2020.0088. Ahead of print.
  
  
• Differentially Regulate Neonatal and Adult Post-Infarct Neovascularisation. Castellan RF, Vitiello M, Vidmar M, Johnstone S, Iacobazzi D, Mellis D, Cathcart B, Thomson AJ, Ruhrberg C, Caputo M, Newby DE, Gray GA, Baker AH, Caporali A, Meloni M. Mir-96 and miR-183 JCI Insight. 2020 Jun 16:134888. doi: 10.1172/jci.insight.134888. Predicted REF score 4*)
  
  
• Successful reconstruction of the right ventricular outflow tract by implantation of thymus stem cell engineered graft in growing swine. Albertario A, Swim MM, Ahmed AM, Iacobazzi D, Yeong M, Madeddu P, Ghorbel MT, Caputo M.  JACC Basic Transl Sci. 2019 Jun 24;4(3):364-384. doi: 10.1016/j.jacbts.2019.02.001. eCollection 2019 Jun Predicted REF score 2-3*
  
  
• Amnion-based scaffold with enhanced strength and biocompatibility for in vivo vascular repair. Tissue Eng Part A.  Swim MM, Albertario A, Iacobazzi D, Caputo M, Ghorbel MT. 2018 Oct 4. doi: 10.1089/ten.TEA.2018.0175.  Predicted REF score 2-3*
  
  
• The distribution, clearance, and brainstem toxicity of panobinostat administered by convection-enhanced delivery.  WGB Singleton, AS Bienemann, M Woolley, D Johnson, O Lewis, SS Gill (2018) Journal of Neurosurgery: Pediatrics 22 (3), 288-296 (2018) (Predicted REF score 3*)
  
  
• Reconstruction of the pulmonary artery by a novel biodegradable conduit engineered with perinatal stem cell-derived vascular smooth muscle cells enables physiological vascular growth in a large animal model of congenital heart disease. Ghorbel MT, Jia H, Swim MM, Iacobazzi D, Albertario A, Zebele C, Holopherne-Doran D, Hollander A, Madeddu P, Caputo M. Biomaterials. 2019 Oct;217:119284. doi: 10.1016/j.biomaterials.2019.119284. Epub 2019 Jun 20. PMID: 31255979 (Predicted REF score 4*)
  
  
• Preparation of viable adult ventricular myocardial slice from large and small mammals. Watson SA, Scigliano M, Bardi I, Ascione R, Terracciano CM, Perbellini F. Nature Protocols 2017; 12: 2623-2639. (Predicted REF score 4*)
  
  
• Thymus derived mesenchymal stem cells for tissue engineering clinical-grade cardiovascular grafts. Tissue Eng Part A. Iacobazzi D, Swim M, Albertario A, Caputo M, Ghorbel MT. 2017 Oct 20. doi: 10.1089/ten.TEA.2017.0290.  Predicted REF score 2-3*
  
  
• Respiratory oscillations in alveolar oxygen tension measured in arterial blood. Formenti, F. Bommakanti, N. Chen, R. Cronin, J. McPeak, H. Holopherne-Doran, D. Hedenstierna, G. Hahn, CEW. Larsson, A. & Farmery, A,D. Sci Rep 7, 7499 (2017). (Predicted REF score 3*)