1979 to 1983

2,512 men aged between 45 and 59 years

Prevalent IHD

• LSHTM chest pain questionaire
• 12 lead ECG
• Family history of IHD

Lifestyle, etc

• Marital state
• Social class and father's social class
• Employment status
• Smoking habit: current habit
• Time since quitting if an ex-smoker
• Diet:questionaire on food frequency
• Alcohol consumption
• 7-day weighed inventory on a 30% sub sample
• Leisure and work activity

Clinical

• Medical history
• Medication and otc preparations
• Height and weight, birthweight
• Skinfolds at four sites
• Lung function: FEV1, and FVC
• Resting BP (random zero sphygmomanometer)

Blood tests (fasting)

• Routine haemotology, including total WCC
• Haemostasis: fibrinogen, plasma viscosity, HTCT, anti-thrombin III
• Lipids: cholesterol, total, VLDL, LDL, HDL, HDL2, HDL3 Triglycerides, total, VLDL, LDL, HDL
• Hormones: testosterone, oestradiol, cortisol insulin
• Blood glucose
• Routine thyroid, liver and kidney function tests

1984 to 1988

Incident IHD - all IHD events between Phases I and II

• Death: deaths coded to ICD 410-414 (9^th revision)
• Myocardial infarction: severe chest pain on the LSHTM questionaire, plus HAA listed events (details in hospital case-notes checked against WHO criteria).
• ECG Myocardial infarction: no Q/QS wave (1-1, 1-2, 1-3) at base line, but major or moderate Q/QS waves (1-1-1 through 1-2-5 plus 1-2-7) on follow-up

Lifestyle, etc

• Marital status, social class and employment status
• Smoking habit
• Diet: questionaire on food frequency, alcohol consumption
• Actvivity: HIPE work activity, Minnesota leisure time activity questionaire

Psycho-social factors

• Social support, job satisfaction 
• Type A behaviour (Jenkins, Bortner and Framingham questionaires) 
• Health attitude inventory (HAI)
• Neuroticism and trait anxiety scales
• General health questionaire (GHQ)

Clinical

• Medical history
• LSHTM intermittent claudication questionnaire
• Medication and otc preparations
• Weight
• Resting BP (random zero sphygmomanometer) and the cold pressor test
• Hearing: Audiometry at four freqencies, noise exposure and noise sensitivity
• Lung function - FEV₁, and FVC

Blood (fasting)

• Haemotalogy: routine tests
• White cell differential count
• Platelets: aggregation in PRP to ADP, thrombin and collagen
• Re-calcification plasma clotting time
• Aggregation in whole blood by an impedance method (350 men)
• Haemostatis: fibrinogen, plasma viscosity, whole blood viscosity (sub-sample)
• Alpha2-macroglobulin and alpha1 - antitrypsin resonace thrombography
• Lipids: cholesterol, triglycerides and HDL-cholesterol, HDL2 and HDL3
• Apolipoproteins AI, AII, B and Lp(a)
• Blood glucose
• Blood Lead, serum and RBC magnesium
• Plasma electrolytes
• Gamma glutamyl transferase
• Routine liver and kidney function tests
• Serum homcysteine
• Plasminogen activator inhibitor activity
• Tissue plasminogen activitor antigen
• Von Willebrand factor antigen
• Fibrin, D-dimer antigen

1989 to 1993

Incident IHD - all IHD events Phases II and III

• IHD Deaths, non-fatal MIs and ECG infarctions as at Phase II
• History of stroke and TIA

Lifestyle, etc

• Marital status
• Social class and employment status
• Smoking habit
• Diet: questionnaire on food frequency
• Alcohol consumption
• Health Attitude Inventory (HAI)
• Social support
• Questionnaire on leisure activities
• Sleep pattern and sleep apnoea

Clinical

• Weight, waist and hip circumference
• Resting blood pressure (random zero sphygmomanometer)
• LSHTM Chest Pain Questionnaire
• Strokes and TIA Questionnnaire
• Medical and otc preparations

Cognitive function (in the home, later)

• AH4, Four choice serial reaction time
• CAMCOG, Rivermead behavioural test
• NART and the General Health Questionnaire (GHQ)

Blood (fasting)

• Lipids: cholesterol and triglycerides
• Routine haemotology including different white cell count
• Haemostasis: fibrinogen, plasma viscosity, von Willebrand Factor
• Factor VII, stressed template bleeding time
• Platelets: platelet aggregation in whole blood by an impedance method
• Shear induced platelet aggregation by a filter technique
• Blood glucose
• Gamma Glutamyl Transferase
• Routine liver and kidney function tests
• Antioxidant enzymes

1993 to 1996

Incident IHD - all IHD events Phases III and IV

• IHD Deaths, non-fatal MIs and ECG infarctions as at Phase II and III
• History of stroke and TIA

Lifestyle, etc

• Employment status
• Smoking habit, alcohol consumption
• Social support
• Leisure activities

Clinical

• Medical history
• Medical and otc preparations
• LSHTM Chest Pain Questionnaire
• Strokes and TIA Questionnnaires
• Height and weight
• Resting blood pressure (random zero sphygmomanometer)
• Audiometry at eight frequencies
• Doppler ultrasound carotid flow recording

Cognitive function

• As in Phase III plus general health questionnaire (GHQ)

Blood (non-fasting)

• Routine haematology
• Fibrinogen and viscosity
• Cholesterol and triglycerides