Dr David Matthews
Senior Lecturer in Virology
School of Cellular and Molecular Medicine,
University of Bristol, Biomedical Sciences Building,
Bristol, BS8 1TD
phone: +44 (0)117 33 12058 (internal 12058)
I am primarily interested in virus host cell interactions with an emphasis on respiratory viruses, in particular adenovirus, but also respiratory syncytial virus, influenza and Hendra virus. I have been looking at how these viruses interact with the host cell using state of the art techniques including laser confocal microscopy, high throughput quantitative mass spectrometry and Deep Sequencing (or Next Generation Sequencing) of virus infected cells. In collaboration with Dr Davidson here at Bristol we also apply these techniques to the study of Dengue virus.
Most recently we have become very interested in the study of novel zoonotic agents such as Hendra, MERS and Ebola. We are at the forefront of developing novel intergrated high throughput methods to study these very dangerous viruses in their natural hosts (bats) to try to understand why they are apparently harmless to their natural host but highly lethal in humans.
In addition, we also use these high throughput approaches to study recombinant gene therapy systems (especially adenovirus based ones). This includes an emphasis on the molecular basis of oncolytic or cancer killing adenoviruses.
- Dowall, S.D., Matthews, D.A., García-Dorival, I., Taylor, I., Kenny, J., Hertz-Fowler, C., Hall, N., Corbin-Lickfett, K., Empig, C., Schlunegger, K., Barr, J.N., Carroll, M.W., Hewson, R. and Hiscox, J.A. (2014) Elucidating variations in the nucleotide sequence of Ebola virus associated with increasing pathogenicity. Genome Biology 15, 540.
- García-Dorival, I., Wu, W., Dowall, S., Armstrong, S., Touzelet, O., Wastling, J., Barr, J.N., Matthews, D., Carroll, M., Hewson, R., and Hiscox, J.A. (2014) Elucidation of the Ebola virus VP24 cellular interactome and disruption of virus biology through targeted inhibition of host cell protein function. J. Proteome. Res. 13, 5120-5135.
- Wynne, J.W., Shiell, B.J., Marsh, G.A., Boyd, V., Harper, J., Heesom, K., Monaghan, P., Zhou, P., Payne, J., Klein, R., Todd, S., Mok, L., Green, D., Bingham, J., Tachedjian, M., Baker, M.L., Matthews, D.A. and Wang, L-F. (2014) Proteomics informed by transcriptomics reveals Hendra virus sensitizes bat cells to TRAIL mediated apoptosis. Genome Biology 15, 532.
- Chiu, H.C., Hannemann, H., Heesom, K.J., Matthews, D.A. and Davidson, A.D. (2014) High-throughput quantitative proteomic analysis of dengue virus type 2 infected a549 cells. PLoS One 9, e93305.
Evans, V.C., Barker, G., Heesom, K.J., Fan, J., Bessant, C., Matthews, D.A. (2012) De novo derivation of proteomes from transcriptomes for transcript and protein identification. Nature Methods 9, 1207-1211.
Wu, W., Tran, K.C., Teng, M.N., Heesom, K.J., Matthews, D.A., Barr, J.N. and Hiscox, J.A. (2012). Solution of the human respiratory syncytial virus NS1 protein interactome highlights multiple effects on host cell biology. J. Virol. 86, 7777 – 7789.
Lam, Y.W., Evans, V.C., Heesom, K.J., Lamond, A.I. and Matthews, D.A. (2010) Proteomic analysis of the nucleolus in adenovirus infected cells. Mol. Cell. Proteomics, 9, 117-130. Highlighted in that months edition.
Emmott, E., Wise, H., Loucaides, E.M., Matthews, D.A., Digard, P. and Hiscox, J. (2010) Quantitative proteomics using SILAC coupled to LC-MS/MS reveals changes in the nucleolar proteome in influenza virus infected cells. J. Proteome Research, 9, 5335-5345.
View all publications for Dr Matthews on his Bristol Research Profile page