Transcription and DNA repair.
Genomes are replicated, repaired, modified and transcribed by a diverse range of protein:protein and protein:nucleic acid complexes.
Research in my laboratory aims to understand the mechanisms by which such complexes cooperate to control the frequency and accuracy with which individual genes are expressed.
We are studying transcription-coupled DNA repair processes and the effect of DNA damage on transcription, using a combination of biochemical and genetic techniques. In collaboration with colleagues from Engineering Maths and Biological Sciences we are also developing synthetic gene regulatory networks that perform designed functions.
Nia Haines, Sarah Miles, Petros Mina, Fiona Preece, and Abigail Smith.
Howan K, Smith AJ, Westblade LF, Joly N, Grange W, Zorman S, Darst SA, Savery NJ, Strick TR. (2012) Initiation of transcription-coupled repair characterized at single-molecule resolution. Nature. 490: 431-434.
Manelyte L, Kim Y-IT, Smith AJ, Smith RM, Savery NJ. (2010) Regulation and Rate Enhancement during Transcription-Coupled DNA Repair. Molecular Cell. 40: 714-724.
Manelyte L, Guy CP, Smith RM, Dillingham MS, McGlynn P, Savery NJ. (2009) The unstructured C-terminal extension of UvrD interacts with UvrB, but is dispensable for nucleotide excision repair. DNA Repair. 8: 1300-1310.
Smith AJ, Szczelkun MD, Savery NJ. (2007) Controlling the motor activity of a transcription-repair coupling factor: autoinhibition and the role of RNA polymerase. Nucleic Acids Research. 35: 1802-1811.