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Publication - Dr Jo Murrell

    Does inflammation induced by ultraviolet B and heat rekindling alter pain-related behaviour in rats?

    Citation

    Smith, MI, Taylor, C, Weerasinghe, NS, Koutsikou, S, Lumb, BM & Murrell, JC, 2016, ‘Does inflammation induced by ultraviolet B and heat rekindling alter pain-related behaviour in rats?’. Veterinary Anaesthesia and Analgesia, vol 43., pp. 579?585

    Abstract

    Objective

    To investigate whether induction of the ultraviolet B and heat rekindling (UVB/HR) model alters burrowing behaviour in rats.

    Study design

    Randomized, blinded, prospective experimental study.

    Animals

    Sixteen adult male Wistar rats weighing 250–300 g.

    Methods

    In the UVB/HR group (n = 8), UV irradiation was delivered to the heel area of the right plantar pelvic limb paw at a dose of 1000 mJ cm−2, using a narrow-band UVB light source. Twenty-four hours later, heat rekindling was performed by placement of a feedback-controlled thermode set at a constant temperature of 45 °C over the area of UVB irradiation for 5 minutes. Both interventions were carried out under pentobarbital anaesthesia. The ‘sham’ group (n = 8) was anaesthetized only. In the burrowing test, rats were housed singly for 2 hours in cages furnished with a burrow filled with sand. The amount of sand remaining in the burrow after 2 hours was weighed and the amount displaced from the burrow calculated. The burrowing test was carried out for two consecutive days prior to UVB irradiation (day 0), on day 1 prior to HR, on days 2 and 3 after UVB exposure and at equivalent time points in the sham group.

    Results

    Rats in the sham group burrowed means (SD) of 2429 (73) g and 2358 (124) g of sand on days −2 and 3, respectively, while those in the UVB/HR group burrowed 2460 (26) and 2419 (58) g on days −2 and 3, respectively. There was no significant effect of treatment on the amount of sand burrowed at any time point.

    Conclusions and clinical relevance

    Pain associated with UVB/HR model induction is below the threshold required to affect rat burrowing behaviour and therefore questions the face validity of UVB/HR as a translational model of inflammatory pain.

    Full details in the University publications repository