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Publication - Dr Timothy Knowles

    Tracing the rate and extent of N and C flow from C-13,N-15-glycine and glutamate into individual de novo synthesised soil amino acids

    Citation

    Knowles, TDJ, Chadwick, DR, Bol, R & Evershed, RP, 2010, ‘Tracing the rate and extent of N and C flow from C-13,N-15-glycine and glutamate into individual de novo synthesised soil amino acids’. Organic Geochemistry, vol 41., pp. 1259-1268

    Abstract

    Mineralisation rates provide valuable information concerning the overall cycling of soil organic N; however, detailed information regarding the pathways preceding the mineralisation of organic substrates remains elusive. We have adopted a molecular approach to open the 'black box' of organic N cycling in soil. Stable isotope probing employing compound-specific isotopic analysis was used to trace the fate of N and C within metabolites central to organic N cycling. In time course experiments, N-15 and C-13 from two dual-labelled amino acid (AA) substrates (U-C-13,N-15-glutamate and U-C-13,N-15-glycine) were followed into AAs biosynthesised de novo. In the majority of cases, highly significant differences (P

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