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Publication - Dr Timothy Knowles

    Dynamic compound-specific stable isotope probing of the soil microbial biomass


    Evershed, R, Charteris, A, Maxfield, P, Bull, I & Knowles, TDJ, 2017, ‘Dynamic compound-specific stable isotope probing of the soil microbial biomass’. in: Kevin R Tate (eds) Microbial biomass: a paradigm shift in terrestrial biogeochemistry. World Scientific Publishing, pp. 169-202


    The concept of following the assimilation of stable, isotopically labelled substrates
    into newly synthesised soil microbial biomass biochemicals (= biomarkers) was
    first demonstrated nearly 20 years ago in studies of unculturable soil methanotrophs (Radajewski et al., 2000; Bull et al., 2000). These landmark papers laid the foundations for an approach that has come to be generally known as stable isotope probing (SIP). The major advantage of SIP in studying the soil microbial
    biomass lies in its effectiveness for investigating unculturable soil microorganisms,
    that is, the vast majority of the soil bacteria, actinomycetes, fungi, algae and

    Full details in the University publications repository