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Publication - Dr Simon Hammann

    Countercurrent chromatographic isolation and purification of 11′-α-tocomonoenol from the vitamin E extract of palm oil

    Citation

    Müller, M, Hammann, S & Vetter, W, 2018, ‘Countercurrent chromatographic isolation and purification of 11′-α-tocomonoenol from the vitamin E extract of palm oil’. Food Chemistry, vol 256., pp. 327-332

    Abstract

    A new vitamin E homologue, α-tocomonoenol was detected in palm oil, but was not isolated in large amounts and with high purity so far. Here we present an easy and fast method to isolate α-tocomonoenol from vitamin E rich nutrient capsules with countercurrent chromatography (CCC). With the solvent system n-hexane – benzotrifluoride– acetonitrile (10:3.5:6.5, v/v/v) about 30mg α-tocomonoenol with a purity of 75% could be enrichedfrom 1g crude sample. Column chromatography with 20% deactivated silica gel and n-hexane – ethyl acetate (95:5, v/v) was performed to gain 5.6mg α-tocomonoenol with a purity of 99.5% according to GC/MS. Structural verification by ⁠1H NMR spectroscopy verified that the double bond was located in 11′-position (11′-α-tocomonoenol). The trace impurity detected in the isolate was identified to be 12′-α-tocomonoenol, a compound previously detected in marine samples.

    Full details in the University publications repository