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Publication - Dr John Day

    Raman spectroscopy of fingernails: A novel tool for evaluation of bone quality?

    Citation

    N.M.Cummins, [V, Day, J, A.Wren, [V, P.Carroll, [V, N.Murphy, [V, P.M.Jakeman, [V & M.R., T, 2010, ‘Raman spectroscopy of fingernails: A novel tool for evaluation of bone quality?’. Spectroscopy, vol 24., pp. 517 - 524

    Abstract

    Raman data from human fingernails has been analysed to determine if a relationship exists between spectral features
    and bone quality. Previous work demonstrated a relationship between the manually determined intensity of the disulphide peak
    (Bone Quality Test; BQT) and osteoporotic fracture. A computer program is now described that automates the BQT determination
    for large sample-sets. In this study the ability of the automated BQT to discriminate between fractures and controls was
    compared to that of bone mineral density (BMD) and biomarkers of bone remodelling. Females aged 18–67 years participated
    in the study (n = 159). Fingernails were analysed using Raman spectroscopy. Lumbar and Femoral BMD was measured by
    dual energy X-ray absorptiometry (DXA). Fasting venepuncture samples were analysed for osteocalcin and CTx (collagen
    C-telopeptides) by electrochemiluminescence. No correlation was found between the BQT and BMD or the biomarkers. The
    BQT and BMD were found to be equally accurate in identifying subjects with a history of fracture (p <0.01) and both outperformed
    osteocalcin and CTx. It appears that keratin and collagen are related structural proteins that require disulphide bonding
    for stability. Therefore, these preliminary results suggest that Raman spectroscopy of keratin may have potential as a diagnostic
    tool for screening bone quality in large populations.

    Full details in the University publications repository