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Publication - Professor Ingeborg Hers

    Aquaporin-1 Regulates Platelet Procoagulant Membrane Dynamics and In Vivo Thrombosis

    Citation

    Agbani, E, Williams, C, Li, Y, van den Bosch, M, Moore, S, Mauroux, A, Hodgson, L, Verkman, AS, Hers, I & Poole, A, 2018, ‘Aquaporin-1 Regulates Platelet Procoagulant Membrane Dynamics and In Vivo Thrombosis’. JCI Insight, vol 3.

    Abstract

    In response to collagen stimulation, platelets use a coordinated system of fluid entry to undergo membrane ballooning, procoagulant-spreading and microvesiculation. We hypothesised that water entry was mediated by the water channel aquaporin-1 (AQP1) and aimed to determine its role in the platelet procoagulant response and thrombosis. We established that human and mouse platelets express AQP1 and localise to internal tubular membrane structures. However, deletion of AQP1 had minimal effects on collagen-induced platelet granule secretion, aggregation or membrane ballooning. Conversely, procoagulant-spreading, microvesiculation, phosphatidylserine exposure and clot formation time were significantly diminished. Furthermore, in vivo thrombus formation after FeCl3 injury to carotid arteries was also markedly suppressed in AQP1-null mice but haemostasis after tail bleeding remained normal. The mechanism involves an AQP1-mediated rapid membrane stretching during procoagulant-spreading but not ballooning, leading to calcium entry through mechanosensitive cation channels and a full procoagulant response. We conclude that AQP1 is a major regulator of the platelet procoagulant response, able to modulate coagulation after injury or pathologic stimuli without affecting other platelet functional responses or normal haemostasis. Clinically effective AQP1 inhibitors may therefore represent a novel class of antiprocoagulant antithrombotics.

    Full details in the University publications repository