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Publication - Professor Clive Orchard

    Caveolin-3 KO Disrupts T-Tububle Structure and Decreases T-Tubular ICa Density in Mouse Ventricular Myocytes


    Bryant, S, Kong, C, Watson, J, Gadeberg, H, Roth, DM, Patel, H, Cannell, M, James, A & Orchard, C, 2018, ‘Caveolin-3 KO Disrupts T-Tububle Structure and Decreases T-Tubular ICa Density in Mouse Ventricular Myocytes’. AJP - Heart and Circulatory Physiology, vol 315., pp. H1101-H1111


    Caveolin-3 (Cav-3) is a protein that has been implicated in t-tubule formation and function in cardiac ventricular myocytes. In cardiac hypertrophy and failure, Cav-3 expression decreases, t-tubule structure is disrupted and excitation-contraction coupling (ECC) is impaired. However, the extent to which the decrease in Cav-3 expression underlies these changes is unclear. We therefore investigated the structure and function of myocytes isolated from the hearts of Cav-3 KO mice. These mice showed cardiac dilatation and decreased ejection fraction in vivo, compared to WT controls. Isolated KO myocytes showed cellular hypertrophy and altered t-tubule structure, and decreased L-type Ca channel (LTCC) current (ICa) density. This decrease in density occurred predominantly in the t-tubules, with no change in total ICa, and was therefore a consequence of the increase in membrane area. Cav-3 KO had no effect on LTCC expression, and C3SD peptide, which mimics the scaffolding domain of Cav-3, had no effect on ICa in KO myocytes. However, inhibiting protein kinase A using H-89 decreased ICa at the surface and t-tubule membranes in both KO and WT myocytes. Cav-3 KO had no significant effect on INCX or Ca release. These data suggest that Cav-3 KO causes cellular hypertrophy thereby decreasing t-tubular ICa density.
    (200 words)

    Full details in the University publications repository