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Publication - Miss Ashley Tyrer

    An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

    Citation

    Dewari, P, Southgate, B, McCarten, K, Monogarov, G, O'Duibhir, E, Quinn, N, Tyrer, A, Leitner, M-C, Plumb, C, Kalantzaki, M, Blin, C, Finch, R, Bressan, R, Morrison, G, Jacobi, A, Behlke, M, von Kriegsheim, A, Tomlinson, S, Krijgsveld, J & Pollard, S, 2018, ‘An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein’. eLife, vol 7.

    Abstract

    CRISPR/Cas9 can be used for precise genetic knock-in of epitope tags into endogenous genes, simplifying experimental analysis of protein function. However, Cas9-assisted epitope tagging in primary mammalian cell cultures is often inefficient and reliant on plasmid-based selection strategies. Here, we demonstrate improved knock-in efficiencies of diverse tags (V5, 3XFLAG, Myc, HA) using co-delivery of Cas9 protein pre-complexed with two-part synthetic modified RNAs (annealed crRNA:tracrRNA) and single-stranded oligodeoxynucleotide (ssODN) repair templates. Knock-in efficiencies of ~5–30%, were achieved without selection in embryonic stem (ES) cells, neural stem (NS) cells, and brain-tumor-derived stem cells. Biallelic-tagged clonal lines were readily derived and used to define Olig2 chromatin-bound interacting partners. Using our novel web-based design tool, we established a 96-well format pipeline that enabled V5-tagging of 60 different transcription factors. This efficient, selection-free and scalable epitope tagging pipeline enables systematic surveys of protein expression levels, subcellular localization, and interactors across diverse mammalian stem cells.

    Full details in the University publications repository