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Publication - Dr Valeska Ting

    Regulation of Scaffold Cell Adhesion Using Artificial Membrane Binding Proteins


    Burke, M, Armstrong, JP, Goodwin, A, Deller, RC, Carter, BM, Harniman, RL, Ginwalla, A, Ting, V, Davis, SA & Perriman, AW, 2017, ‘Regulation of Scaffold Cell Adhesion Using Artificial Membrane Binding Proteins’. Macromolecular Bioscience, vol 17.


    The rapid pace of development in biotechnology has placed great importance on controlling cell-material interactions. In practice, this involves attempting to decouple the contributions from adhesion molecules, cell membrane receptors, and scaffold surface chemistry and morphology, which is extremely challenging. Accordingly, a strategy is presented in which different chemical, biochemical, and morphological properties of 3D biomaterials are systematically varied to produce novel scaffolds with tuneable cell affinities. Specifically, cationized and surfactant-conjugated proteins, recently shown to have non-native membrane affinity, are covalently attached to 3D scaffolds of collagen or carboxymethyl-dextran, yielding surface-functionalized 3D architectures with predictable cell immobilization profiles. The artificial membrane-binding proteins enhance cellular adhesion of human mesenchymal stem cells (hMSCs) via electrostatic and hydrophobic binding mechanisms. Furthermore, functionalizing the 3D scaffolds with cationized or surfactant-conjugated myoglobin prevents a slowdown in proliferation of seeded hMSCs cultured for seven days under hypoxic conditions.

    Full details in the University publications repository