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Publication - Dr Shane Windsor

    Phospholipase C-zeta-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injectiond

    Citation

    Swann, K, Windsor, S, Campbell, K, Elgmati, K, Nomikos, M, Zernicka-Goetz, M, Amso, N, Lai, A, Thomas, A & Graham, C, 2012, ‘Phospholipase C-zeta-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injectiond’. Fertility and Sterility, vol 97., pp. 742-747

    Abstract

    Objective: To evaluate the imaging of cytoplasmic movements in human oocytes as a potential method to monitor the pattern of Ca2+ oscillations during activation.

    Design: Test of a laboratory technique.

    Setting: University medical school research laboratory.

    Patient(s): Donated unfertilized human oocytes from intracytoplasmic sperm injection (ICSI) cycles.

    Intervention(s): Microinjection of oocytes with phospholipase C (PLC) zeta (zeta) cRNA and a Ca2+-sensitive fluorescent dye.

    Main Outcome Measure(s): Simultaneous detection of oocyte cytoplasmic movements using particle image velocimetry (PIV) and of Ca2+ oscillations using a Ca2+-sensitive fluorescent dye.

    Result(s): Microinjection of PLC zeta cRNA into human oocytes that had failed to fertilize after ICSI resulted in the appearance of prolonged Ca2+ oscillations. Each transient Ca2+ concentration change was accompanied by a small coordinated movement of the cytoplasm that could be detected using PIV analysis.

    Conclusion(s): The occurrence and frequency of cytoplasmic Ca2+ oscillations, a critical parameter in activating human zygotes, can be monitored by PIV analysis of cytoplasmic movements. This simple method provides a novel, noninvasive approach to determine in real time the occurrence and frequency of Ca2+ oscillations in human zygotes. (Fertil Steril (R) 2012; 97: 742-7. (C) 2012 by American Society for Reproductive Medicine.)

    Full details in the University publications repository