Browse/search for people

Publication - Dr Helene Ledru

    Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

    Citation

    Huang, K, Parmeggiani, F, Ledru, H, Hollingsworth, K, Pons, JM, Marchesi, A, Both, P, Mattey, AP, Pallister, E, Bulmer, GS, van Munster, JM, Turnbull, WB, Galan, C & Flitsch, SL, 2019, ‘Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases’. Organic and Biomolecular Chemistry, vol 17., pp. 5920-5924

    Abstract

    Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

    Full details in the University publications repository