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Professor David Murphy
Professor David Murphy
Professor of Experimental Medicine
Area of research
The Molecular Neueroendocrinology Research Group uses gene discovery and transfer techniques to study the neuronal regulation of the cardiovascular system in health and disease. Interests have focussed on hypothalamic neurons involved in the maintenance of plasma osmolality, and the integration of autonomic cardiovascular signalling by brainstem (nucleus of the solitary tract; NTS) neurons (in collaboration with Dr Julian Paton, Dept of Physiology, University of Bristol).
We are using microarray, differential screening and yeast two-hybrid methodologies to identify genes expressed in hypothalamic and brainstem neurons, and genes which alter their pattern of expression following physiological challenges. The hypotheses that emerge from the analysis of expression information must be tested with genetic methods involving the transfer of genes into model organisms. We have accumulated considerable experience with the generation and analysis of transgenic models; both germline systems mediated by the microinjection of fertilised one-cell eggs with cloned DNA fragments, and somatic systems mediated by the stereotaxic injection of Adenoviral gene transfer vectors into specific brain regions. Current strategies involve combining gene discovery with precise real-time physiological measurements.
Activities / Findings
- Microarray and gene transfer studies on the homeostatic plasticity of hypothalamic neurons
- Autophagy and the aetiology of inherited central diabetes insipidus
- Molecular mechanisms of the regulated secretion of hypothalamic peptide hormones
MSc in Molecular Neuroscience
- nitric oxide
- Diabetes insipidus
Processes and functions
- blood volume
- blood pressure
- adenoviral vectors
- in situ hybridisation
- Western blotting
- Northern blots
- Hendy, E, Marvar, P, Cruise, T, Walas, D, DeCicco, D, Vadigepalli, R, Schwaber, J, Waki, H, Murphy, D & Paton, JFR, 2016, Systemic leukotriene B4 receptor antagonism lowers arterial blood pressure and improves autonomic function in the spontaneously hypertensive rat. Journal of Physiology, vol 594., pp. 5975-5989
- Lozić, M, Tasić, T, Martin, A, Greenwood, M, Šarenac, O, Hindmarch, C, Paton, JF, Murphy, D & Japundžić-Žigon, N, 2016, Over-expression of V1a receptors in PVN modulates autonomic cardiovascular control. Pharmacological Research, vol 114., pp. 185-195
- Greenwood, MP, Greenwood, M, Mecawi, ADS, Antunes-Rodrigues, J, Paton, JFR & Murphy, D, 2016, Rasd1, a small G protein with a big role in the hypothalamic response to neuronal activation. Molecular Brain, vol 9.
- Greenwood, MP, Greenwood, M, Gillard, BT, Loh, SY, Paton, JFR & Murphy, D, 2016, Epigenetic control of the vasopressin promoter explains physiological ability to regulate vasopressin transcription in dehydration and salt loading states in the rat. Journal of Neuroendocrinology, vol 28.
- Konopacka, A, Qiu, J, Yao, ST, Greenwood, MP, Greenwood, M, Lancaster, T, Inoue, W, Mecawi, AdS, Vechiato, FMV, Lima, JBMd, Coletti, R, Hoe, SZ, Martin, AM, Lee, J, Joseph, M, Hindmarch, C, Paton, J, Antunes-Rodrigues, J, Bains, J & Murphy, D, 2015, Osmoregulation Requires Brain Expression of the Renal Na-K-2Cl Cotransporter NKCC2. The Journal of Neuroscience, vol 35., pp. 5144-5155
- Greenwood, MP, Mecawi, AS, Hoe, SZ, Mustafa, MR, Johnson, KR, Al-Mahmoud, GA, Elias, LLK, Paton, JFR, Antunes-Rodrigues, J, Gainer, H, Murphy, D & Hindmarch, CCT, 2015, A comparison of physiological and transcriptome responses to water deprivation and salt loading in the rat supraoptic nucleus. American Journal of Physiology Integrative Comp Physiol, vol 308., pp. R559-R568
- Greenwood, M, Greenwood, MP, Mecawi, AS, Loh, SY, Rodrigues, JA, Paton, JFR & Murphy, D, 2015, Transcription factor CREB3L1 mediates cAMP and glucocorticoid regulation of arginine vasopressin gene transcription in the rat hypothalamus. Molecular Brain, vol 8.
- Greenwood, MP, Greenwood, M, Paton, JFR & Murphy, D, 2015, Control of Polyamine Biosynthesis by Antizyme Inhibitor 1 is Important for Transcriptional Regulation of Arginine Vasopressin in the Male Rat Hypothalamus. Endocrinology., pp. en20151074
- Greenwood, M, Greenwood, MP, Paton, JFR & Murphy, D, 2015, Transcription Factor CREB3L1 Regulates Endoplasmic Reticulum Stress Response Genes in the Osmotically Challenged Rat Hypothalamus. PloS one, vol 10., pp. e0124956
- Konopacka, A, Greenwood, M, Loh, SY, Paton, J & Murphy, D, 2015, RNA binding protein Caprin-2 is a pivotal regulator of the central osmotic defense response. eLife, vol 4.
Networks & contacts
- Dr Julian Paton - Bristol
- Prof Nina Japunzic - Belgrade Serbia
- Dr Tom Cunningham - The University of Texas Health Science Center at San Antonio
- Dr Jeff Tasker - Tulane University New Orleans
- Prof Ciro Isidoro - Università "A. Avogadro" in Novara Italy
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