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Details for Professor David Murphy

Personal details
Name Professor David Murphy
Job title Professor of Experimental Medicine
Department Bristol Medical School (THS) University of Bristol
Contact details This expert can be contacted via the University of Bristol Public Relations Office.

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work+44 (0)117 331 8092
email:
Qualifications BSc(Edin), PhD(Lond)
Professional details
Keywords vasopressin
oxytocin
hypothalamus
transcriptosome
proteome
transgenics
hypertension
Areas of expertise The Molecular Neueroendocrinology Research Group uses gene discovery and transfer techniques to study the neuronal regulation of the cardiovascular system in health and disease. Interests have focussed on hypothalamic neurons involved in the maintenance of plasma osmolality, and the integration of autonomic cardiovascular signalling by brainstem (nucleus of the solitary tract; NTS) neurons (in collaboration with Dr Julian Paton.

We are using microarray, differential screening and yeast two-hybrid methodologies to identify genes expressed in hypothalamic and brainstem neurons, and genes which alter their pattern of expression following physiological challenges. We have accumulated considerable experience with the generation and analysis of transgenic models; both germline systems mediated by the microinjection of fertilised one-cell eggs with cloned DNA fragments, and somatic systems mediated by the stereotaxic injection of Adenoviral gene transfer vectors into specific brain regions. Current strategies involve combining gene discovery with precise real-time physiological measurements.