LOCUS       pTYF-2xGfaABC1D-       11496 bp    DNA     circular     15-OCT-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|496242791|
COMMENT     VNTDBDATE|496242833|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTYF-2xGfaABC1D-tTA|
COMMENT     VNTAUTHORNAME|Sergey|
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FEATURES             Location/Qualifiers
     promoter        26..711
                     /vntifkey="29"
                     /label=GfaABC1D(26-711)
     exon            789..1848
                     /vntifkey="61"
                     /label=Gal4p65(789-1848)
     polyA_signal    1898..2280
                     /vntifkey="25"
                     /label=SV40pA(1898-2280)
     protein_bind    2284..2399
                     /vntifkey="31"
                     /label=5xGAL4BS(2284-2399)
     promoter        2399..3086
                     /vntifkey="29"
                     /label=GfaABC1D(2399-3086)
     exon            3092..4097
                     /vntifkey="61"
                     /label=tTA(3092-4097)
BASE COUNT     3038 a      2814 c      2948 g      2695 t         1 others
ORIGIN
        1 ggcctaggga taacagggta atgattaata acatatcctg gtgtggagta ggggacgctg 
       61 ctctgacaga ggctcggggg cctgagctgg ctctgtgagc tggggaggag gcagacagcc 
      121 aggccttgtc tgcaagcaga cctggcagca ttgggctggc cgccccccag ggcctcctct 
      181 tcatgcccag tgaatgactc accttggcac agacacaatg ttcggggtgg gcacagtgcc 
      241 tgcttcccgc cgcaccccag cccccctcaa atgccttccg agaagcccat tgagcagggg 
      301 gcttgcattg caccccagcc tgacagcctg gcatcttggg ataaaagcag cacagccccc 
      361 taggggctgc ccttgctgtg tggcgccacc ggcggtggag aacaaggctc tattcagcct 
      421 gtgcccagga aaggggatca ggggatgccc aggcatggac agtgggtggc agggggggag 
      481 aggagggctg tctgcttccc agaagtccaa ggacacaaat gggtgagggg agagctctcc 
      541 ccatagctgg gctgcggccc aaccccaccc cctcaggcta tgccaggggg tgttgccagg 
      601 ggcacccggg catcgccagt ctagcccact ccttcataaa gccctcgcat cccaggagcg 
      661 agcagagcca gagcaggttg gagaggagac gcatcacctc cgctgctcgc gctagcgatt 
      721 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctg atctaagcct 
      781 cctgaaagat gaagctactg tcttctatcg aacaagcatg cgatatttgc cgacttaaaa 
      841 agctcaagtg ctccaaagaa aaaccgaagt gcgccaagtg tctgaagaac aactgggagt 
      901 gtcgctactc tcccaaaacc aaaaggtctc cgctgactag ggcacatctg acagaagtgg 
      961 aatcaaggct agaaagactg gaacagctat ttctactgat ttttcctcga gaagaccttg 
     1021 acatgatttt gaaaatggat tctttacagg atataaaagc attgttaaca ggattatttg 
     1081 tacaagataa tgtgaataaa gatgccgtca cagatagatt ggcttcagtg gagactgata 
     1141 tgcctctaac attgagacag catagaataa gtgcgacatc atcatcggaa gagagtagta 
     1201 acaaaggtca aagacagttg actgtatcgc cggaattggg atccatggtg tttccttctg 
     1261 ggcagatctc aaaccaggcc ctggccttag caccgtcctc tgccccagtc cttgcccaga 
     1321 ccatggtccc ttcctcagcc atggtacctc tggctcagcc cccagctcct gccccagttc 
     1381 taaccccggg tcctccccag tccctgtctg cacctgttcc aaagagcacc caggctgggg 
     1441 aaggcacgct gtcggaagcc ctgctgcacc tgcagtttga tgctgatgaa gacttggggg 
     1501 ccttgcttgg caacagcaca gacccaggag tgttcacaga cctggcatct gtggacaact 
     1561 cagagtttca gcagctcctg aaccagggtg tgtccatgtc tcactccaca gctgagccca 
     1621 tgctgatgga gtaccctgaa gctataactc gcctggtgac agggtcccag aggccccctg 
     1681 acccagctcc cacacccctg gggacctcgg ggcttcccaa tggtctctcc ggagatgaag 
     1741 acttctcctc cattgcggac atggacttct ctgctctgct gagtcagatc agctccagcg 
     1801 gccaatctag actgcagctc gacctcgagg gggggcccgg taccaggtaa gtgtacccaa 
     1861 ttcgccctat agtgagtcgt attacaattc actcgatcgc ccttcccaac agttgcgcag 
     1921 cctgaatggc gaatggagat ccaattttta agtgtataat gtgttaaact actgattcta 
     1981 attgtttgtg tattttagat tcacagtccc aaggctcatt tcaggcccct cagtcctcac 
     2041 agtctgttca tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 
     2101 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 
     2161 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     2221 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 
     2281 cgcgctagcc ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc 
     2341 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagact ctagagggac 
     2401 gcgtaacata tcctggtgtg gagtagggga cgctgctctg acagaggctc gggggcctga 
     2461 gctggctctg tgagctgggg aggaggcaga cagccaggcc ttgtctgcaa gcagacctgg 
     2521 cagcattggg ctggccgccc cccagggcct cctcttcatg cccagtgaat gactcacctt 
     2581 ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt cccgccgcac cccagccccc 
     2641 ctcaaatgcc ttccgagaag cccattgagc agggggcttg cattgcaccc cagcctgaca 
     2701 gcctggcatc ttgggataaa agcagcacag ccccctaggg gctgcccttg ctgtgtggcg 
     2761 ccaccggcgg tggagaacaa ggctctattc agcctgtgcc caggaaaggg gatcagggga 
     2821 tgcccaggca tggacagtgg gtggcagggg gggagaggag ggctgtctgc ttcccagaag 
     2881 tccaaggaca caaatgggtg aggggagagc tctccccata gctgggctgc ggcccaaccc 
     2941 caccccctca ggctatgcca gggggtgttg ccaggggcac ccgggcatcg ccagtctagc 
     3001 ccactccttc ataaagccct cgcatcccag gagcgagcag agccagagca ggttggagag 
     3061 gagacgcatc acctccgctg ctcgcggatc catgtctaga ttagataaaa gtaaagtgat 
     3121 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact 
     3181 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc 
     3241 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt 
     3301 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt 
     3361 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta 
     3421 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc 
     3481 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga 
     3541 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt 
     3601 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct 
     3661 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag 
     3721 ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag ggcctgctcg atctcccgga 
     3781 cgacgacgcc cccgaagagg cggggctggc ggctccgcgc ctgtcctttc tccccgcggg 
     3841 acacacgcgc agactgtcga cggccccccc gaccgatgtc agcctggggg acgagctcca 
     3901 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga 
     3961 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta 
     4021 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat 
     4081 tgacgagtac ggtgggtagc tcgaggctgc agggcggccg catcgattag ggataacagg 
     4141 gtaatggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 
     4201 aaagaaaagg ggggactgga agggctaatt cactcccagg gtctctctgg ttagaccaga 
     4261 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 
     4321 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 
     4381 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 
     4441 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 
     4501 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 
     4561 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 
     4621 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
     4681 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctgtgcg ggagaacgga 
     4741 gttctattat gactcaaatc agtctcccca agcattcggg gatcagagtt tttaaggata 
     4801 acttagtgtg tagggggcca gtgagttgga gatgaaagcg tagggagtcg aaggtgtcct 
     4861 tttgcgccga gtcagttcct gggtgggggc cacaagatcg gatgagccag tttatcaatc 
     4921 cgggggtgcc agctgatcca tggagtgcag ggtctgcaaa atatctcaag cactgattga 
     4981 tcttaggttt tacaatagtg atgttacccc aggaacaatt tggggaaggt cagaatcttg 
     5041 tagcctgtag ctgcatgact cctaaaccat aatttctttt ttgttttttt ttttttattt 
     5101 ttgagacagg gtctcactct gtcacctagg ctggagtgca gtggtgcaat cacagctcac 
     5161 tgcagcctca acgtcgtaag ctcaagcgat cctcccacct cagcctgcct ggtagctgag 
     5221 actacaagcg acgccccagt taatttttgt atttttggta gaggcagcgt tttgccgtgt 
     5281 ggccctggct ggtctcgaac tcctgggctc aagtgatcca gcctcagcct cccaaagtgc 
     5341 tgggacaacc ggggccagtc actgcacctg gccctaaacc ataatttcta atcttttggc 
     5401 taatttgtta gtcctacaaa ggcagtctag tccccaggca aaaagggggt ttgtttcggg 
     5461 aaagggctgt tactgtcttt gtttcaaact ataaactaag ttcctcctaa acttagttcg 
     5521 gcctacaccc aggaatgaac aaggagagct tggaggttag aagcacgatg gaattggtta 
     5581 ggtcagatct ctttcactgt ctgagttata attttgcaat ggtggttcaa agactgcccg 
     5641 cttctgacac cagtcgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 
     5701 tattggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     5761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     5821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     5881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     5941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     6001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     6061 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag 
     6121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     6181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     6241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     6301 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 
     6361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     6421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     6481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     6541 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     6601 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     6661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     6721 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     6781 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     6841 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     6901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     6961 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     7021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     7081 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     7141 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     7201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     7261 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     7321 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     7381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     7441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     7501 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     7561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     7621 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 
     7681 aaaaataggc gtatcacgag gccctttcgt cttcaagaac tgcctcgcgc gtttcggtga 
     7741 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 
     7801 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 
     7861 cgcagccatg acccagtcac gtagcgatag cggagtgtac tggcttaact atgcggcatc 
     7921 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 
     7981 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 
     8041 atcggtgcgg gcctcttcgc tattacgcca gcgcggggag gcagagattg cagtaagctg 
     8101 agatcgcagc actgcactcc agcctgggcg acagagtaag actctgtctc aaaaataaaa 
     8161 taaataaatc aatcagatat tccaatcttt tcctttattt atttatttat tttctatttt 
     8221 ggaaacacag tccttcctta ttccagaatt acacatatat tctatttttc tttatatgct 
     8281 ccagtttttt ttagaccttc acctgaaatg tgtgtataca aaatctaggc cagtccagca 
     8341 gagcctaaag gtaaaaaata aaataataaa aaataaataa aatctagctc actccttcac 
     8401 atcaaaatgg agatacagct gttagcatta aataccaaat aacccatctt gtcctcaata 
     8461 attttaagcg cctctctcca ccacatctaa ctcctgtcaa aggcatgtgc cccttccggg 
     8521 cgctctgctg tgctgccaac caactggcat gtggactctg cagggtccct aactgccaag 
     8581 ccccacagtg tgccctgagg ctgccccttc cttctagcgg ctgcccccac tcggctttgc 
     8641 tttccctagt ttcagttact tgcgttcagc caaggtctga aactaggtgc gcacagagcg 
     8701 gtaagactgc gagagaaaga gaccagcttt acagggggtt tatcacagtg caccctgaca 
     8761 gtcgtcagcc tcacaggggg tttatcacat tgcaccctga cagtcgtcag cctcacaggg 
     8821 ggtttatcac agtgcaccct tacaatcatt ccatttgatt cacaattttt ttagtctcta 
     8881 ctgtgcctaa cttgtaagtt aaatttgatc agaggtgtgt tcccagaggg gaaaacagta 
     8941 tatacagggt tcagtactat cgcatttcag ggtggcccga gagctgcatc cggagtatct 
     9001 agatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 
     9061 acccccgccc attgacgtca ataatgactg atgttcccat agtaacgcca atagggactt 
     9121 tccattgacg ctaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 
     9181 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtactcta gaaggtctat 
     9241 ataagcagag ctcgtttagt gaaccgttta tactacttat ctggtctctc tggttagacc 
     9301 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 
     9361 gcttgccttg agtgctcaaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 
     9421 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga 
     9481 cttgaaagcg aaagtaaagc cagaggagat ctctcgacgc aggactcggc ttgctgaagc 
     9541 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg 
     9601 aggctagaag gagagagatg ggtgcgagag cgtcggtatt aagcggggga gaattagata 
     9661 aatgggaaaa aattcggtta aggccagggg gaaagaaaca atataaacta aaacatatag 
     9721 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctttta gagacatcag 
     9781 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 
     9841 ttagatcatt atataataca atagcagtcc tctattgtgt gcatcaaagg atagatgtaa 
     9901 aagacaccaa ggaagcctta gataagatag aggaagagca aaacaaaagt aagaaaaagg 
     9961 cacagcaagc agcagctgac acaggaaaca acagccaggt cagccaaaat taccctatag 
    10021 tgcagaacct ccaggggcaa atggtacatc aggccatatc acctagaact ttaaatgcat 
    10081 gggtaaaagt agtagaagag aaggctttca gcccagaagt aatacccatg ttttcagcat 
    10141 tatcagaagg agccacccca caagatttaa ataccatgct aaacacagtg gggggacatc 
    10201 aagcagccat gcaaatgtta aaagagacca tcaatgagga agctgcagaa tgggatagat 
    10261 tgcatccagt gcatgcaggg cctattgcac caggccagat gagagaacca aggggaagtg 
    10321 acatagcagg aactactagc aaattaagag aacaatttgg aaataataaa acaataatct 
    10381 ttaagcaatc ctcaggaggg gacccagaaa ttgtaacgca cagttttaat tgtggagggg 
    10441 aatttttcta ctgtaattca acacaactgt ttaatagtac ttggtttaat agtacttgga 
    10501 gtactgaagg gtcaaataac actgaaggaa gtgacacaat cacactccca tgcagaataa 
    10561 aacaatttat aaacatgtgg caggaagtag gaaaagcaat gtatgcccct cccatcagtg 
    10621 gacaaattag atgttcatca aatattactg ggctgctatt aacaagagat ggtggtaata 
    10681 acaacaatgg gtccgagatc ttcagacctg gaggaggcga tatgagggac aattggagaa 
    10741 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg 
    10801 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg 
    10861 ggttcttggg agcagcagga agcactatgg gcgcacggtc aatgacgctg acggtacagg 
    10921 ccagacaatt attgtctgat atagtgcagc agcagaacaa tttgctgagg gctattgagg 
    10981 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa acagctccag gcaagaatcc 
    11041 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa 
    11101 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac 
    11161 agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat tacacaagct 
    11221 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat 
    11281 tggaattaga taaatgggct tgcggccgga attcacaaat ggcagtattc atccacaatt 
    11341 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 
    11401 caacagacat acaaactaaa gaattacaaa aacaaattac taaaaattcn aaaattttcg 
    11461 ggtttattac agggacagca gagatccact ttgggc 
//
