LOCUS       pTYF-mCMV/GfaABC       11273 bp    DNA     circular     25-JUN-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|486035569|
COMMENT     VNTDBDATE|486035569|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pTYF-mCMV/GfaABC1D-EGFP|
COMMENT     VNTAUTHORNAME|Sergey|
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COMMENT                                   -24.3926 -8.34611 42.5095 4.27096 0.974079 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (3234)" "@N (@S)" 5 15.2336 
COMMENT                                   -33.1043 -8.3198 47.5762 4.42082 0.974079 
COMMENT                                   #297#) 
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COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (8754)" "@N (@S)" 5 -14.2798 
COMMENT                                   3.48466 9.71542 7.04245 4.42082 0.974079 
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COMMENT               #302=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -2200 100) 
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FEATURES             Location/Qualifiers
     exon            complement(424..1483)
                     /vntifkey="61"
                     /label=Gal4BDp65
     promoter        complement(1558..1622)
                     /vntifkey="29"
                     /label=minCMV
     misc_binding    1650..1741
                     /vntifkey="20"
                     /label=5xGal4BS
     promoter        1747..2428
                     /vntifkey="29"
                     /label=GfaABC1D
     exon            2460..3255
                     /vntifkey="61"
                     /label=EGFP
     enhancer        3282..3900
                     /vntifkey="9"
                     /label=WPRE
     polyA_signal    complement(73..417)
                     /vntifkey="25"
                     /label=SV40pA
BASE COUNT     2900 a      2776 c      2903 g      2693 t         1 others
ORIGIN
        1 ggcctaggga taacagggta atgatatctc tgaccgctag catcgtcgac ccgggatccg 
       61 cggactagtc gcgttaagat acattgatga gtttggacaa accacaacta gaatgcagtg 
      121 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 
      181 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 
      241 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga 
      301 tcatgaacag actgtgagga ctgaggggcc tgaaatgagc cttgggactg tgaatctaaa 
      361 atacacaaac aattagaatc agtagtttaa cacattatac acttaaaaat tggatctact 
      421 tattcaagat cggccgctgg agctgatctg actcagcaga gcagagaagt ccatgtccgc 
      481 aatggaggag aagtcttcat ctccggagag accattggga agccccgagg tccccagggg 
      541 tgtgggagct gggtcagggg gcctctggga ccctgtcacc aggcgagtta tagcttcagg 
      601 gtactccatc agcatgggct cagctgtgga gtgagacatg gacacaccct ggttcaggag 
      661 ctgctgaaac tctgagttgt ccacagatgc caggtctgtg aacactcctg ggtctgtgct 
      721 gttgccaagc aaggccccca agtcttcatc agcatcaaac tgcaggtgca gcagggcttc 
      781 cgacagcgtg ccttccccag cctgggtgct ctttggaaca ggtgcagaca gggactgggg 
      841 aggacccggg gttagaactg gggcaggagc tgggggctga gccagaggta ccatggctga 
      901 ggaagggacc atggtctggg caaggactgg ggcagaggac ggtgctaagg ccagggcctg 
      961 gtttgagatc tgcccagaag gaaacaccat ggtgagctct gcagaagctt ctagaattcc 
     1021 agcccgggcg gatcccaatt ccggcgatac agtcaactgt ctttgacctt tgttactact 
     1081 ctcttccgat gatgatgtcg cacttattct atgctgtctc aatgttagag gcatatcagt 
     1141 ctccactgaa gccaatctat ctgtgacggc atctttattc acattatctt gtacaaataa 
     1201 tcctgttaac aatgctttta tatcctgtaa agaatccatt ttcaaaatca tgtcaaggtc 
     1261 ttctcgagga aaaatcagta gaaatagctg ttccagtctt tctagccttg attccacttc 
     1321 tgtcagatgt gccctagtca gcggagacct tttggttttg ggagagtagc gacactccca 
     1381 gttgttcttc agacacttgg cgcacttcgg tttttctttg gagcacttga gctttttaag 
     1441 tcggcaaata tcgcatgctt gttcgataga agacagtagc ttcatctttc aggaggctag 
     1501 cttagatccc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcgct 
     1561 agcggatctg acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc 
     1621 taacgcgcta gccttgcatg cctgcaggtc ggagtactgt cctccgagcg gagtactgtc 
     1681 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc 
     1741 cgagcggaga ctctagaggg acgcgtaaca tatcctggtg tggagtaggg gacgctgctc 
     1801 tgacagaggc tcgggggcct gagctggctc tgtgagctgg ggaggaggca gacagccagg 
     1861 ccttgtctgc aagcagacct ggcagcattg ggctggccgc cccccagggc ctcctcttca 
     1921 tgcccagtga atgactcacc ttggcacaga cacaatgttc ggggtgggca cagtgcctgc 
     1981 ttcccgccgc accccagccc ccctcaaatg ccttccgaga agcccattga gcagggggct 
     2041 tgcattgcac cccagcctga cagcctggca tcttgggata aaagcagcac agccccctag 
     2101 gggctgccct tgctgtgtgg cgccaccggc ggtggagaac aaggctctat tcagcctgtg 
     2161 cccaggaaag gggatcaggg gatgcccagg catggacagt gggtggcagg gggggagagg 
     2221 agggctgtct gcttcccaga agtccaagga cacaaatggg tgaggggaga gctctcccca 
     2281 tagctgggct gcggcccaac cccaccccct caggctatgc cagggggtgt tgccaggggc 
     2341 acccgggcat cgccagtcta gcccactcct tcataaagcc ctcgcatccc aggagcgagc 
     2401 agagccagag caggttggag aggagacgca tcacctccgc tgctcgcact agtgccacca 
     2461 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 
     2521 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 
     2581 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 
     2641 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc 
     2701 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 
     2761 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 
     2821 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 
     2881 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 
     2941 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 
     3001 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 
     3061 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 
     3121 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg 
     3181 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat 
     3241 ccaccggatc tagataactg atcataatca gccataccac gcggccgctc ccgttatttg 
     3301 cgctctgttc ctgttaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 
     3361 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 
     3421 gctattactt cccgtacggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 
     3481 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 
     3541 gacgcaaccc ccactggttg gggcattgcc accacctatc aactcctttc cgggactttc 
     3601 gctttccccc tccctattgc cacggcggaa ctcattgccg cctgccttgc ccgctgctgg 
     3661 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc 
     3721 tttccatggc tgctcgcctg tgttgccaac tggattctgc gcgggacgtc cttctgctac 
     3781 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggttctgcgg 
     3841 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctat 
     3901 cgattaggga taacagggta atggtacctt taagaccaat gacttacaag gcagctgtag 
     3961 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccagggtc 
     4021 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
     4081 taagcctcaa taaagctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 
     4141 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 
     4201 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 
     4261 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 
     4321 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 
     4381 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 
     4441 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 
     4501 ctgtgcggga gaacggagtt ctattatgac tcaaatcagt ctccccaagc attcggggat 
     4561 cagagttttt aaggataact tagtgtgtag ggggccagtg agttggagat gaaagcgtag 
     4621 ggagtcgaag gtgtcctttt gcgccgagtc agttcctggg tgggggccac aagatcggat 
     4681 gagccagttt atcaatccgg gggtgccagc tgatccatgg agtgcagggt ctgcaaaata 
     4741 tctcaagcac tgattgatct taggttttac aatagtgatg ttaccccagg aacaatttgg 
     4801 ggaaggtcag aatcttgtag cctgtagctg catgactcct aaaccataat ttcttttttg 
     4861 tttttttttt tttatttttg agacagggtc tcactctgtc acctaggctg gagtgcagtg 
     4921 gtgcaatcac agctcactgc agcctcaacg tcgtaagctc aagcgatcct cccacctcag 
     4981 cctgcctggt agctgagact acaagcgacg ccccagttaa tttttgtatt tttggtagag 
     5041 gcagcgtttt gccgtgtggc cctggctggt ctcgaactcc tgggctcaag tgatccagcc 
     5101 tcagcctccc aaagtgctgg gacaaccggg gccagtcact gcacctggcc ctaaaccata 
     5161 atttctaatc ttttggctaa tttgttagtc ctacaaaggc agtctagtcc ccaggcaaaa 
     5221 agggggtttg tttcgggaaa gggctgttac tgtctttgtt tcaaactata aactaagttc 
     5281 ctcctaaact tagttcggcc tacacccagg aatgaacaag gagagcttgg aggttagaag 
     5341 cacgatggaa ttggttaggt cagatctctt tcactgtctg agttataatt ttgcaatggt 
     5401 ggttcaaaga ctgcccgctt ctgacaccag tcgctgcatt aatgaatcgg ccaacgcgcg 
     5461 gggagaggcg gtttgcgtat tggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     5521 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     5581 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     5641 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     5701 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     5761 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     5821 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 
     5881 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     5941 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     6001 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     6061 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     6121 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     6181 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     6241 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     6301 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     6361 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     6421 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     6481 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     6541 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     6601 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     6661 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     6721 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     6781 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     6841 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     6901 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     6961 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     7021 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     7081 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     7141 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     7201 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     7261 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     7321 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     7381 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 
     7441 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaactgc 
     7501 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 
     7561 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 
     7621 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtactgg 
     7681 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 
     7741 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc 
     7801 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcg cggggaggca 
     7861 gagattgcag taagctgaga tcgcagcact gcactccagc ctgggcgaca gagtaagact 
     7921 ctgtctcaaa aataaaataa ataaatcaat cagatattcc aatcttttcc tttatttatt 
     7981 tatttatttt ctattttgga aacacagtcc ttccttattc cagaattaca catatattct 
     8041 atttttcttt atatgctcca gtttttttta gaccttcacc tgaaatgtgt gtatacaaaa 
     8101 tctaggccag tccagcagag cctaaaggta aaaaataaaa taataaaaaa taaataaaat 
     8161 ctagctcact ccttcacatc aaaatggaga tacagctgtt agcattaaat accaaataac 
     8221 ccatcttgtc ctcaataatt ttaagcgcct ctctccacca catctaactc ctgtcaaagg 
     8281 catgtgcccc ttccgggcgc tctgctgtgc tgccaaccaa ctggcatgtg gactctgcag 
     8341 ggtccctaac tgccaagccc cacagtgtgc cctgaggctg ccccttcctt ctagcggctg 
     8401 cccccactcg gctttgcttt ccctagtttc agttacttgc gttcagccaa ggtctgaaac 
     8461 taggtgcgca cagagcggta agactgcgag agaaagagac cagctttaca gggggtttat 
     8521 cacagtgcac cctgacagtc gtcagcctca cagggggttt atcacattgc accctgacag 
     8581 tcgtcagcct cacagggggt ttatcacagt gcacccttac aatcattcca tttgattcac 
     8641 aattttttta gtctctactg tgcctaactt gtaagttaaa tttgatcaga ggtgtgttcc 
     8701 cagaggggaa aacagtatat acagggttca gtactatcgc atttcagggt ggcccgagag 
     8761 ctgcatccgg agtatctaga tggagttccg cgttacataa cttacggtaa atggcccgcc 
     8821 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgactgatg ttcccatagt 
     8881 aacgccaata gggactttcc attgacgcta atgggagttt gttttggcac caaaatcaac 
     8941 gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg 
     9001 tactctagaa ggtctatata agcagagctc gtttagtgaa ccgtttatac tacttatctg 
     9061 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 
     9121 gcttaagcct caataaagct tgccttgagt gctcaaagta gtgtgtgccc gtctgttgtg 
     9181 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 
     9241 tggcgcccga acagggactt gaaagcgaaa gtaaagccag aggagatctc tcgacgcagg 
     9301 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca 
     9361 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cggtattaag 
     9421 cgggggagaa ttagataaat gggaaaaaat tcggttaagg ccagggggaa agaaacaata 
     9481 taaactaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg 
     9541 ccttttagag acatcagaag gctgtagaca aatactggga cagctacaac catcccttca 
     9601 gacaggatca gaagaactta gatcattata taatacaata gcagtcctct attgtgtgca 
     9661 tcaaaggata gatgtaaaag acaccaagga agccttagat aagatagagg aagagcaaaa 
     9721 caaaagtaag aaaaaggcac agcaagcagc agctgacaca ggaaacaaca gccaggtcag 
     9781 ccaaaattac cctatagtgc agaacctcca ggggcaaatg gtacatcagg ccatatcacc 
     9841 tagaacttta aatgcatggg taaaagtagt agaagagaag gctttcagcc cagaagtaat 
     9901 acccatgttt tcagcattat cagaaggagc caccccacaa gatttaaata ccatgctaaa 
     9961 cacagtgggg ggacatcaag cagccatgca aatgttaaaa gagaccatca atgaggaagc 
    10021 tgcagaatgg gatagattgc atccagtgca tgcagggcct attgcaccag gccagatgag 
    10081 agaaccaagg ggaagtgaca tagcaggaac tactagcaaa ttaagagaac aatttggaaa 
    10141 taataaaaca ataatcttta agcaatcctc aggaggggac ccagaaattg taacgcacag 
    10201 ttttaattgt ggaggggaat ttttctactg taattcaaca caactgttta atagtacttg 
    10261 gtttaatagt acttggagta ctgaagggtc aaataacact gaaggaagtg acacaatcac 
    10321 actcccatgc agaataaaac aatttataaa catgtggcag gaagtaggaa aagcaatgta 
    10381 tgcccctccc atcagtggac aaattagatg ttcatcaaat attactgggc tgctattaac 
    10441 aagagatggt ggtaataaca acaatgggtc cgagatcttc agacctggag gaggcgatat 
    10501 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
    10561 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
    10621 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cacggtcaat 
    10681 gacgctgacg gtacaggcca gacaattatt gtctgatata gtgcagcagc agaacaattt 
    10741 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaaaca 
    10801 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
    10861 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
    10921 taataaatct ctggaacaga tttggaataa catgacctgg atggagtggg acagagaaat 
    10981 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
    11041 gaatgaacaa gaattattgg aattagataa atgggcttgc ggccggaatt cacaaatggc 
    11101 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag 
    11161 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattactaa 
    11221 aaattcnaaa attttcgggt ttattacagg gacagcagag atccactttg ggc 
//
