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Return to Histological Staining Techniques


TECHNIQUE: STAINS FOR:
Alcian blue acid mucins (proteoglycans)
Alcian blue/PAS acid and neutral mucins
Alcian blue/safranine different types of mast cell
Aldehyde fuchsin pancreatic islet cells
Alizarin red calcium deposits
Barrett's pituitary cells
Carbol chromotrope eosinophils
Congo red amyloid
Csaba  see alcian blue/safranine
Dunn/Thompson haemoglobin casts
EVG (elastin van Gieson) elastin (see Miller's)
Fontana melanin (see Masson/Fontana)
Fouchet bile pigments
Giemsa blood cells, parasites
Gomori hexamine silver (also called methenamine silver) basement membranes
Gordon & Sweets reticulin
Gram or Gram/Twort bacteria
Grimelius argyrophil cells
Grocott hexamine silver fungi
HVG (haematoxylin van Gieson) collagen, muscle
HID/AB (high iron diamine/alcian blue) sulphated and carboxylated mucins
Leishman blood cells, parasites
Luxol fast blue/cresyl violet myelin, nissl substance (CNS)
MGP (methyl green pyronin) DNA, RNA
MPTAH (Mallory's phosphotungstic acid haematoxylin) all tissue components (metachromatic)
MSB (martius scarlet blue) fibrin, connective tissue
Macchiavello rickettsiae, viral inclusions
Masson/Fontana melanin
Miller's elastin
Naumenko/Feigin astrocytes
Oil red O fat (frozen sections)
PAS (periodic acid Schiff) carbohydrates
Perl's Prussian blue ferric iron
Permanganate bleach removes melanin
Phloxine/tartrazine viral inclusions
Rubeanic acid copper
Safranine O/fast green cartilage
Schmorl lipofuscin
Sirius red amyloid
Solochrome cyanin myelin (non CNS)
Toluidine blue mast cells
Toluidine blue amyloid
Turnbull ferrous iron
Von Kossa calcium
Warthin/Starry Tyzzer's disease, spirochaetes
Wade/Fite leprosy bacilli
Weigert/van Gieson see HVG
ZN (Ziehl/Neelsen) TB bacilli