Areas of expertise
I focus on type II restriction endonucleases which require two copies of their recognition site in order to cleave DNA. These proteins are of interest as models for DNA-protein interactions and long-range communications between distant DNA sites. Below is an overview of the specific enzymes I study: -Long rage communications: mechanisms by which restriction enzymes can mediate long range communications. -SfiI: a tetramer which binds to and cleaves two sites concertedly. It is a type IIf endonuclease. -SgrAI: a type IIf endonuclease. It exists as a monomer/dimer equilibrium in solution and is thought to tetramise on DNA. -BspMI: a tetrameric type IIs endonuclease which binds to and cleaves two sites concertedly. It is unusual because most type IIs endonucleases are monomeric in solution. -FokI: a type IIs endonuclease which binds to two sites but cleaves only one. -BbvCI: a heterodimeric type II endonuclease which binds to an asymmetric recognition sequence.