Xenopus Tadpole Research

Outlinehatchling tadpole

We use embryos and young tadpoles of the frog Xenopus to ask how nervous systems work at the cellular level, develop to form the correct connections, and are organised to allow animals to behave. Our methods include: analysis of behaviour, whole-cell patch recording, neuron imaging, network modelling. See examples of our projects.

tadpole swimmingThe young Xenopus tadpole provides a very simple model animal with limited behaviour. It can swim either spontaneously or when touched anywhere on the body. A pineal eye detects dimming which speeds up swimming. The tadpole stops swimming when the head bumps into solid objects and the tadpole sticks to things with mucus secreted by a cement gland on the head. If the tadpole is held it can make stronger struggling movements.

This simple behaviour is coordinated by an extremely basic nervous circuit with very few differentiated classes of neuron. For example, at the time of hatching, the spinal cord appears to have only 8 different classes of neuron but the spinal tadpole can still swim. Simple methods for testing the behavioural responses of normal and genetically manipulated tadpoles are given.

New BBSRC Project: “Cross-modality integration of sensory signals leading to initiation of locomotion”  This project will start next year and we will be recruiting a new Post-Doctoral Research assistant

BBSRC Project (2009 to 2013): A neuronal network generating flexible locomotor behaviour in a simple vertebrate: studies on function and embryonic self-assembly

Other Projects:

Publications

Collaborators

  • Dr Wen-Chang Li, (Royal Society University Research Fellow, School of Biology, University of St Andrews): Physiology and anatomy of CNS neurons.
  • Prof Roman Borisyuk, (Centre for Theoretical and Computational Neuroscience, University of Plymouth): Modelling developmant of neuronal circuits..
  • Dr Ervin Wolf, (University of Debrecen, Hungary): Computer modelling.

Postgraduate Opportunities

 We are always looking for potential postgraduate students with an genuine interest in studying how the nervous system of frog tadpoles develops and functions. Contact Dr Steve Soffe or Prof Alan Roberts

Links to related sites