Dr Charlotte Spink
Current position: LRF Post-Doctoral Research Assistant in the Immunogenetics Group, Department of Pathology and Microbiology, University of Bristol (appointed Jan 2004)
BSc. (Hons) Zoology, Cardiff University PhD University of West of England
We are currently involved in a study investigating polymorphisms and mutations in the NF-kB/IkB pathway in multiple myeloma, with an aim to looking at associations with disease status and progression-free/overall survival. For polymorphism detection we use Induced Heteroduplex Generator (IHG) technology, a novel genotyping method, which can unequivocally distinguish homozygotes from heterozygotes and mutation screening has been carried out using a combination of denaturing HPLC (WAVE Ô) and DNA sequencing.
Publications and Posters;
Spink CF, Keen LJ, Rollinson S, Bidwell JL and Morgan G. Use of Induced Heteroduplex Generator (IHG) technology for genotyping of Tumour Necrosis Factor (TNF) region microsatellites in patients with B cell lymphoproliferative disorders. Proceedings of the American Journal of Cancer Research, Vol 43, (abstract 3815), March 2002.
Spink CF, Adamson PJ, Mensah FK, Law G, Davies FE, Bidwell JL, Keen LJ, Morgan GJ. The haplotypic structure of the TNF alpha locus and its impact on aetiology of multiple myeloma. Journal of the American Society of Haematology. (abstract 5081) 2002.
Spink CF, Keen LJ, Mensah FK, Adamson PJ, Law G, Bidwell JL and Morgan GJ. Polymorphism in the interleukin-10 promoter predicts susceptibility to Diffuse large B cell lymphoma but not Follicular centre cell lymphoma. Proceedings of the American Journal of Cancer Research, 1st Edition. Vol 44 (abstract 2589) March 2003.
Spink CF, Keen LJ, Middleton PG, and Bidwell JL. Discrimination of suballeles present at the TNFd microsatellite locus using induced heteroduplex analysis. (2004) Genes and Immunity. 5: 76-79.
Genetic polymorphism in the NF-kB/IkB complex in Multiple Myeloma. Multiple Myeloma Forum Meeting, London, 3rd February 2005 (Oral presentation).
Morgan GJ, Adamson PJ, Mensah FK, Spink CF, Law GR, Keen LJ, Roman E, Davies FE, Rollinson S, Child JA and Bidwell JL. (2005) Haplotypes in the Tumour Necrosis Factor region and Myeloma. British Journal of Haematology (in press)
Spink CF, Gray LC, Davies FE, Morgan GJ and Bidwell JL. (2005). Haplotypic structure across the IkBagene and association with Multiple Myeloma. (in prep)