Dr Russell Garland
Postdoctoral Research Associate, BCH Haematology and Bone Marrow Transplantation Research Group, Department of Pathology and Microbiology, University of Bristol (appointed December 1999)
BSc (Hons), PhD (Bristol), CBiol, MIBiol
My PhD studies at the University of Bristol resulted in a thesis entitled “The ex vivo expansion of human CD8 cytotoxic T lymphocytes to Herpes Simplex Virus”. I then spent three months working in the laboratory of Professor Hans-Georg Rammensee (Department of Immunology, University of Tubingen, German) in order to learn how to perform HLA-peptide binding and IFNg ELISPOT assays. Subsequently, I pursued my interest in cellular immunity to viruses of the herpes family by devising a study investigating the reconstitution of immunity to Cytomegalovirus (CMV) after stem cell transplantation (SCT) with my principal investigator, Dr Colin Steward.
Using flow cytometry and functional assays, we have been evaluating immune reconstitution in a cohort of patients post-SCT. Through collaboration with the Molecular Virology laboratory of the Bristol Health Protection Agency (Drs David Carrington and Barry Vipond) we have compared the kinetics of CMV reactivation episodes in this cohort with SCT type and immune parameters. Colin and I also collaborate with the HPA in studies of Adenovirus post-SCT.
My current laboratory co-workers are Dr Paraskevi Diamanti and Sarah Groves. Paraskevi is a postdoctoral scientist working jointly between our lab and the group of Dr Allison Blair and Derwood Pamphilon to work up clinical scale isolation of CMV-specific T cells for treatment of CMV infection post-SCT. Sarah is a research technician involved in our immune reconstitution studies and in monitoring levels of the T cell depleting antibody CAMPATH in patients post-SCT.
Further details are given under:
Al-Shanti N, Steward CG, Garland RJ, Rowbottom AW. Investigation of alpha nascent polypeptide-associated complex functions in a human CD8( ) T cell ex vivo expansion model using antisense oligonucleotides. Immunology. 2004 Jul;112(3):397-403.
Al-Shanti NA, Steward CG, Garland RJ. Optimization of functional efficacy of phosphorothioate-modified oligonucleotides in a human CD8 T-cell ex vivo expansion model. Scand J Immunol. 2003 Oct;58(4):462-70.
Garland RJ, El-Shanti N, West SE, Hancock JP, Goulden NJ, Steward CG, Rowbottom AW. Human CD8 CTL recognition and in vitro lysis of herpes simplex virus-infected cells by a non-MHC restricted mechanism. Scand J Immunol. 2002 Jan;55(1):61-9.
Rowbottom AW, Garland RJ, Lepper MW, Kaneria SS, Goulden NJ, Oakhill A, Steward CG. Functional analysis of the CD8 CD57 cell population in normal healthy individuals and matched unrelated T-cell-depleted bone marrow transplant recipients. Br J Haematol. 2000 Aug;110(2):315-21.